Conformational Conversion Enhances Cellular Uptake of F Base Double-Strand-Conjugated Oligonucleotides

doi:10.1021/acs.analchem.0c00614

HFCAS OpenIR

	Conformational Conversion Enhances Cellular Uptake of F Base Double-Strand-Conjugated Oligonucleotides
	Bai, Huarong 3; Jin, Cheng 3,4; Zou, Jianmei 3; Wang, Ruowen 4; Fu, Ting 1,2; Tan, Weihong 3,4
	2020-08-04
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
通讯作者	Fu, Ting(futinghnu@hnu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn)
摘要	Artificial bases have emerged as a useful tool to expand genetic alphabets and biomedical applications of oligonucleotides. Herein, we reported that the conformation conversion enhances cellular uptake of hydrophobic 3,5-bis(trifluoromethyl)benzene (F) base double-strand-conjugated oligonucleotides. The formation of the F base double-strand caged the hydrophobic F base in the duplex strand, thus preventing F base from interacting with cells to some extent. However, upon conversion of F base double-strand-conjugated oligonucleotide to F base single-strand-conjugated oligonucleotide, F bases then were allowed to interact with cells by stronger hydrophobic interactions, followed by cellular uptake. The results were concluded as a pairing-induced cage effect of F base and have the potential for the construction of stimuli-responsive cellular uptake of functional nucleic acids.
DOI	10.1021/acs.analchem.0c00614
关键词[WOS]	SEMISYNTHETIC ORGANISM ; EVOLUTION
收录类别	SCI
语种	英语
资助项目	China NSFC[NSFC 21521063] ; China NSFC[NSFC 21327009]
项目资助者	China NSFC
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000558761500028
出版者	AMER CHEMICAL SOC
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/102916
专题	中国科学院合肥物质科学研究院
通讯作者	Fu, Ting; Tan, Weihong
作者单位	1.Univ Chinese Acad Sci, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China 3.Hunan Univ, Coll Chem & Chem Engn, Aptamer Engn Ctr Hunan Prov,Coll Biol, Mol Sci & Biomed Lab MBL,State Key Lab Chemo Bios, Changsha 410082, Hunan, Peoples R China 4.Shanghai Jiao Tong Univ, Inst Mol Med IMM, Renji Hosp, State Key Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China
推荐引用方式 GB/T 7714	Bai, Huarong,Jin, Cheng,Zou, Jianmei,et al. Conformational Conversion Enhances Cellular Uptake of F Base Double-Strand-Conjugated Oligonucleotides[J]. ANALYTICAL CHEMISTRY,2020,92.
APA	Bai, Huarong,Jin, Cheng,Zou, Jianmei,Wang, Ruowen,Fu, Ting,&Tan, Weihong.(2020).Conformational Conversion Enhances Cellular Uptake of F Base Double-Strand-Conjugated Oligonucleotides.ANALYTICAL CHEMISTRY,92.
MLA	Bai, Huarong,et al."Conformational Conversion Enhances Cellular Uptake of F Base Double-Strand-Conjugated Oligonucleotides".ANALYTICAL CHEMISTRY 92(2020).