Exosome-transferred long non-coding RNA ASMTL-AS1 contributes to malignant phenotypes in residual hepatocellular carcinoma after insufficient radiofrequency ablation

doi:10.1111/cpr.12795

HFCAS OpenIR

	Exosome-transferred long non-coding RNA ASMTL-AS1 contributes to malignant phenotypes in residual hepatocellular carcinoma after insufficient radiofrequency ablation
	Ma, Dening 1,2,3; Gao, Xinyi 1,4,5; Liu, Zhuo 1,2,3; Lu, Xingang 1,2,3; Ju, Haixing 1,2,3; Zhang, Ning 6
	2020-07-28
发表期刊	CELL PROLIFERATION
ISSN	0960-7722
通讯作者	Ju, Haixing(juhx@zjcc.org.cn) ; Zhang, Ning(zning818@163.com)
摘要	Objectives Long non-coding RNAs (lncRNAs) are emerging RNA regulators in cancer progression, including in hepatocellular carcinoma (HCC). Recently, insufficient radiofrequency ablation (RFA) has been reported to lead to recurrence and metastasis of residual HCC tumours. Herein, we aimed to the role of ASMTL-AS1 in residual HCC after insufficient RFA. Materials and methods In vitro insufficient RFA model was simulated in Huh7 cells and subsequently named Huh7-H cells. In vitro and in vivo assays were conducted to investigate ASMTL-AS1 function in HCC. Results LncRNA ASMTL-AS1 low expressed in normal human liver was found to be highly expressed in HCC tissues and further increased in tumours after insufficient RFA. ASMTL-AS1 expression was related to stage, metastasis and prognosis in HCC. Huh7-H possessed higher ASMTL-AS1 level and more aggressive than Huh7 cells. ASMTL-AS1 contributed to the malignancy of HCC cells both in vitro and in vivo. Mechanistically, ASMTL-AS1 was trans-activated by MYC and promoted NLK expression to activate YAP signalling via sequestering miR-342-3p in HCC. Interestingly, ASMTL-AS1 could be wrapped by exosomes and then convey malignancy through NLK/YAP axis between cells even in residual HCC after insufficient RFA. Conclusions Exosomal ASMTL-AS1 aggravates the malignancy in residual HCC after insufficient RFA via miR-342-3p/NLK/YAP signalling, opening a new road for the treatment of HCC and the prevention of recurrence or metastasis of residual HCC after insufficient RFA.
关键词	ASMTL-AS1 exosomes HCC NLK YAP nuclear translocation
DOI	10.1111/cpr.12795
关键词[WOS]	NEMO-LIKE KINASE ; CELL-PROLIFERATION ; CANCER STATISTICS ; PHOSPHORYLATION ; RECURRENCE ; CERNA
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81702371]
项目资助者	National Natural Science Foundation of China
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000552876800001
出版者	WILEY
引用统计	被引频次：38[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/102950
专题	中国科学院合肥物质科学研究院
通讯作者	Ju, Haixing; Zhang, Ning
作者单位	1.Chinese Acad Sci, Inst Canc & Basic Med ICBM, 1 Banshan East Rd, Hangzhou 310022, Peoples R China 2.Univ Chinese Acad Sci, Canc Hosp, Dept Colorectal Surg, 1 Banshan East Rd, Hangzhou 310022, Peoples R China 3.Zhejiang Canc Hosp, Dept Colorectal Surg, 1 Banshan East Rd, Hangzhou 310022, Peoples R China 4.Univ Chinese Acad Sci, Canc Hosp, Dept Radiol, 1 Banshan East Rd, Hangzhou 310022, Peoples R China 5.Zhejiang Canc Hosp, Dept Radiol, 1 Banshan East Rd, Hangzhou 310022, Peoples R China 6.Fudan Univ, Canc Hosp, Dept Hepat Surg, Shanghai Canc Ctr, 270 Dong An Rd, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Ma, Dening,Gao, Xinyi,Liu, Zhuo,et al. Exosome-transferred long non-coding RNA ASMTL-AS1 contributes to malignant phenotypes in residual hepatocellular carcinoma after insufficient radiofrequency ablation[J]. CELL PROLIFERATION,2020.
APA	Ma, Dening,Gao, Xinyi,Liu, Zhuo,Lu, Xingang,Ju, Haixing,&Zhang, Ning.(2020).Exosome-transferred long non-coding RNA ASMTL-AS1 contributes to malignant phenotypes in residual hepatocellular carcinoma after insufficient radiofrequency ablation.CELL PROLIFERATION.
MLA	Ma, Dening,et al."Exosome-transferred long non-coding RNA ASMTL-AS1 contributes to malignant phenotypes in residual hepatocellular carcinoma after insufficient radiofrequency ablation".CELL PROLIFERATION (2020).