Integrated Analyses Identify Immune-Related Signature Associated with Qingyihuaji Formula for Treatment of Pancreatic Ductal Adenocarcinoma Using Network Pharmacology and Weighted Gene Co-Expression Network

doi:10.1155/2020/7503605

HFCAS OpenIR

	Integrated Analyses Identify Immune-Related Signature Associated with Qingyihuaji Formula for Treatment of Pancreatic Ductal Adenocarcinoma Using Network Pharmacology and Weighted Gene Co-Expression Network
	Qian, Xiang 1,2,3; Chen, Zhuo 1,2,3; Chen, Sha Sha 4; Liu, Lu Ming 5; Zhang, Ai Qin 1,2,3
	2020-05-20
发表期刊	JOURNAL OF IMMUNOLOGY RESEARCH
ISSN	2314-8861
通讯作者	Zhang, Ai Qin(15867100095@sina.cn)
摘要	The study aimed to clarify the potential immune-related targets and mechanisms of Qingyihuaji Formula (QYHJ) against pancreatic cancer (PC) through network pharmacology and weighted gene co-expression network analysis (WGCNA). Active ingredients of herbs in QYHJ were identified by the TCMSP database. Then, the putative targets of active ingredients were predicted with SwissTargetPrediction and the STITCH databases. The expression profiles of GSE32676 were downloaded from the GEO database. WGCNA was used to identify the co-expression modules. Besides, the putative targets, immune-related targets, and the critical module genes were mapped with the specific disease to select the overlapped genes (OGEs). Functional enrichment analysis of putative targets and OGEs was conducted. The overall survival (OS) analysis of OGEs was investigated using the Kaplan-Meier plotter. The relative expression and methylation levels of OGEs were detected in UALCAN, human protein atlas (HPA), Oncomine, DiseaseMeth version 2.0 and, MEXPRESS database, respectively. Gene set enrichment analysis (GSEA) was conducted to elucidate the key pathways of highly-expressed OGEs further. OS analyses found that 12 up-regulated OGEs, including CDK1, PLD1, MET, F2RL1, XDH, NEK2, TOP2A, NQO1, CCND1, PTK6, CTSE, and ERBB2 that could be utilized as potential diagnostic indicators for PC. Further, methylation analyses suggested that the abnormal up-regulation of these OGEs probably resulted from hypomethylation, and GSEA revealed the genes markedly related to cell cycle and proliferation of PC. This study identified CDK1, PLD1, MET, F2RL1, XDH, NEK2, TOP2A, NQO1, CCND1, PTK6, CTSE, and ERBB2 might be used as reliable immune-related biomarkers for prognosis of PC, which may be essential immunotherapies targets of QYHJ.
DOI	10.1155/2020/7503605
关键词[WOS]	TRADITIONAL CHINESE MEDICINE ; LIVER METASTASES ; CANCER ; EXPRESSION ; INHIBITION ; INVASION
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81673742] ; project of Young talents of traditional Chinese Medicine of Zhejiang Provincial[2017ZQ006]
项目资助者	National Natural Science Foundation of China ; project of Young talents of traditional Chinese Medicine of Zhejiang Provincial
WOS研究方向	Immunology
WOS类目	Immunology
WOS记录号	WOS:000537950300002
出版者	HINDAWI LTD
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/103096
专题	中国科学院合肥物质科学研究院
通讯作者	Zhang, Ai Qin
作者单位	1.Chinese Acad Sci, Inst Canc & Basic Med ICBM, Hangzhou, Peoples R China 2.Univ Chinese Acad Sci, Canc Hosp, Hangzhou, Peoples R China 3.Zhejiang Canc Hosp, Hangzhou, Peoples R China 4.Taizhou Canc Hosp, Dept Tradit Chinese Med, Taizhou, Zhejiang, Peoples R China 5.Fudan Univ, Dept Integrat Oncol, Shanghai Canc Ctr, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Qian, Xiang,Chen, Zhuo,Chen, Sha Sha,et al. Integrated Analyses Identify Immune-Related Signature Associated with Qingyihuaji Formula for Treatment of Pancreatic Ductal Adenocarcinoma Using Network Pharmacology and Weighted Gene Co-Expression Network[J]. JOURNAL OF IMMUNOLOGY RESEARCH,2020,2020.
APA	Qian, Xiang,Chen, Zhuo,Chen, Sha Sha,Liu, Lu Ming,&Zhang, Ai Qin.(2020).Integrated Analyses Identify Immune-Related Signature Associated with Qingyihuaji Formula for Treatment of Pancreatic Ductal Adenocarcinoma Using Network Pharmacology and Weighted Gene Co-Expression Network.JOURNAL OF IMMUNOLOGY RESEARCH,2020.
MLA	Qian, Xiang,et al."Integrated Analyses Identify Immune-Related Signature Associated with Qingyihuaji Formula for Treatment of Pancreatic Ductal Adenocarcinoma Using Network Pharmacology and Weighted Gene Co-Expression Network".JOURNAL OF IMMUNOLOGY RESEARCH 2020(2020).