Construction of Bispecific Aptamer-Drug Conjugate by a Hybrid Chemical and Biological Approach

doi:10.1021/acs.bioconjchem.0c00071

HFCAS OpenIR

	Construction of Bispecific Aptamer-Drug Conjugate by a Hybrid Chemical and Biological Approach
	Sun, Yang 1,2; Gao, Fei 1,2; Yang, Cai 1,2; Li, Yingying3,4 ; Jin, Cheng 3,4; Xie, Sitao 3,4; Lv, Cheng 1; Ding, Ding 1; Han, Da 1; Li, Juan 1; Wang, Ruowen 1,2; Tan, Weihong 1,2,3,4,5
	2020-05-01
发表期刊	BIOCONJUGATE CHEMISTRY
ISSN	1043-1802
通讯作者	Wang, Ruowen(wangwrw@sjtu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn)
摘要	Bispecific aptamer-drug conjugates (BsApDC) may improve the efficacy of drugs by enhancing cellular internalization and targeted delivery. Nevertheless, the synthesis of single-molecular BsApDC has not yet been reported, and it could be thwarted by synthetic challenges. Herein we report a general approach to synthesize a BsApDC hybridized chemical and biological method. Primers incorporated with 5-Fluorouracil (5-FU), 10-Hydroxycamptothecin, and Maleimidocaproyl-valinecitrulline-p-aminobenzoyloxycarbonyl-monomethyl auristatin E(vcMMAE) were prepared by chemical synthesis, which were converted to corresponding ApDCs efficiently by enzymatic reaction. Biological studies revealed that BsApDC binds with target cells with enhanced internalization and better inhibitory activity, demonstrating the potential of BsApDCs for targeted tumor therapy.
DOI	10.1021/acs.bioconjchem.0c00071
关键词[WOS]	NEXT-GENERATION ; DNA ; DELIVERY ; GROWTH ; CELLS
收录类别	SCI
语种	英语
资助项目	NSFC[NSFC 21877079] ; NSFC[NSFC 81803068] ; ZJNSF[LY16B020001] ; NIH[GM R35 127130] ; NSF[1645215]
项目资助者	NSFC ; ZJNSF ; NIH ; NSF
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
WOS记录号	WOS:000537398400008
出版者	AMER CHEMICAL SOC
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/103106
专题	中国科学院合肥物质科学研究院
通讯作者	Wang, Ruowen; Tan, Weihong
作者单位	1.Shanghai Jiao Tong Univ, Renji Hosp, Inst Mol Med, Sch Med,State Key Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China 3.Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn,Mol Sci & Biomed Lab MBL, Changsha 410082, Hunan, Peoples R China 4.Hunan Univ, Aptamer Engn Ctr Hunan Prov, Changsha 410082, Hunan, Peoples R China 5.Univ Chinese Acad Sci, Chinese Acad Sci, Canc Hosp, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yang,Gao, Fei,Yang, Cai,et al. Construction of Bispecific Aptamer-Drug Conjugate by a Hybrid Chemical and Biological Approach[J]. BIOCONJUGATE CHEMISTRY,2020,31.
APA	Sun, Yang.,Gao, Fei.,Yang, Cai.,Li, Yingying.,Jin, Cheng.,...&Tan, Weihong.(2020).Construction of Bispecific Aptamer-Drug Conjugate by a Hybrid Chemical and Biological Approach.BIOCONJUGATE CHEMISTRY,31.
MLA	Sun, Yang,et al."Construction of Bispecific Aptamer-Drug Conjugate by a Hybrid Chemical and Biological Approach".BIOCONJUGATE CHEMISTRY 31(2020).