Knowledge Management System of Hefei Institute of Physical Science,CAS
Equipping Natural Killer Cells with Specific Targeting and Checkpoint Blocking Aptamers for Enhanced Adoptive Immunotherapy in Solid Tumors | |
Zhang, Da1,4; Zheng, Youshi4; Lin, Ziguo4; Liu, Xiaolong4; Li, Juan1,2,3; Yang, Huanghao1; Tan, Weihong2,3 | |
2020-05-12 | |
发表期刊 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION |
ISSN | 1433-7851 |
通讯作者 | Li, Juan(lijuan@fzu.edu.cn) ; Yang, Huanghao(hhyang@fzu.edu.cn) |
摘要 | Herein, we propose an aptamer-equipping strategy to generate specific, universal and permeable (SUPER) NK cells for enhanced immunotherapy in solid tumors. NK cells were chemically equipped with TLS11a aptamer targeting HepG2 cells and PDL1-specific aptamer without genetic alteration. The dual aptamer-equipped NK cells exhibited high specificity to tumor cells, resulting in higher cytokine secretion and apoptosis/necrosis compared to parental or single aptamer-equipped NK cells. Interestingly, dual aptamer-equipped NK cells induced remarkable upregulation of PDL1 expression in HepG2 cells, enhancing checkpoint blockade. Furthermore, in vivo intravital imaging demonstrated high infiltration of aptamer-equipped NK cells into deep tumor region, leading to enhanced therapeutic efficacy in solid tumors. This work offers a straightforward chemical strategy to equip NK cells with aptamers, holding considerable potential for enhanced adoptive immunotherapy in solid tumors. |
关键词 | aptamers immunology natural killer cells PD1 PDL1 checkpoint blockade solid tumors |
DOI | 10.1002/anie.202002145 |
关键词[WOS] | CANCER-IMMUNOTHERAPY ; TRANSFER THERAPY ; DNA APTAMER |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Natural Science Foundation of China[91959102] ; Natural Science Foundation of China[21635002] ; Natural Science Foundation of China[61805041] ; Natural Science Foundation of Fujian Province of China[2017J06004] ; Shanghai Rising-Star Program[19QA1405400] ; Program for Changjiang Scholars and Innovative Research Team in University[IRT15R11] |
项目资助者 | Natural Science Foundation of China ; Natural Science Foundation of Fujian Province of China ; Shanghai Rising-Star Program ; Program for Changjiang Scholars and Innovative Research Team in University |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000531691900001 |
出版者 | WILEY-V C H VERLAG GMBH |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/103282 |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Li, Juan; Yang, Huanghao |
作者单位 | 1.Fuzhou Univ, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Coll Chem,State Key Lab Photocatalysis Energy & E, Fuzhou 350116, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med, Hangzhou 310022, Zhejiang, Peoples R China 3.Univ Chinese Acad Sci, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 4.Fujian Med Univ, Mengchao Hepatobiliary Hosp, United Innovat Mengchao Hepatobiliary Technol Key, Fuzhou 350025, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Da,Zheng, Youshi,Lin, Ziguo,et al. Equipping Natural Killer Cells with Specific Targeting and Checkpoint Blocking Aptamers for Enhanced Adoptive Immunotherapy in Solid Tumors[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2020. |
APA | Zhang, Da.,Zheng, Youshi.,Lin, Ziguo.,Liu, Xiaolong.,Li, Juan.,...&Tan, Weihong.(2020).Equipping Natural Killer Cells with Specific Targeting and Checkpoint Blocking Aptamers for Enhanced Adoptive Immunotherapy in Solid Tumors.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. |
MLA | Zhang, Da,et al."Equipping Natural Killer Cells with Specific Targeting and Checkpoint Blocking Aptamers for Enhanced Adoptive Immunotherapy in Solid Tumors".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020). |
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