Equipping Natural Killer Cells with Specific Targeting and Checkpoint Blocking Aptamers for Enhanced Adoptive Immunotherapy in Solid Tumors

doi:10.1002/anie.202002145

HFCAS OpenIR

	Equipping Natural Killer Cells with Specific Targeting and Checkpoint Blocking Aptamers for Enhanced Adoptive Immunotherapy in Solid Tumors
	Zhang, Da 1,4; Zheng, Youshi 4; Lin, Ziguo 4; Liu, Xiaolong 4; Li, Juan 1,2,3; Yang, Huanghao 1; Tan, Weihong 2,3
	2020-05-12
发表期刊	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN	1433-7851
通讯作者	Li, Juan(lijuan@fzu.edu.cn) ; Yang, Huanghao(hhyang@fzu.edu.cn)
摘要	Herein, we propose an aptamer-equipping strategy to generate specific, universal and permeable (SUPER) NK cells for enhanced immunotherapy in solid tumors. NK cells were chemically equipped with TLS11a aptamer targeting HepG2 cells and PDL1-specific aptamer without genetic alteration. The dual aptamer-equipped NK cells exhibited high specificity to tumor cells, resulting in higher cytokine secretion and apoptosis/necrosis compared to parental or single aptamer-equipped NK cells. Interestingly, dual aptamer-equipped NK cells induced remarkable upregulation of PDL1 expression in HepG2 cells, enhancing checkpoint blockade. Furthermore, in vivo intravital imaging demonstrated high infiltration of aptamer-equipped NK cells into deep tumor region, leading to enhanced therapeutic efficacy in solid tumors. This work offers a straightforward chemical strategy to equip NK cells with aptamers, holding considerable potential for enhanced adoptive immunotherapy in solid tumors.
关键词	aptamers immunology natural killer cells PD1 PDL1 checkpoint blockade solid tumors
DOI	10.1002/anie.202002145
关键词[WOS]	CANCER-IMMUNOTHERAPY ; TRANSFER THERAPY ; DNA APTAMER
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of China[91959102] ; Natural Science Foundation of China[21635002] ; Natural Science Foundation of China[61805041] ; Natural Science Foundation of Fujian Province of China[2017J06004] ; Shanghai Rising-Star Program[19QA1405400] ; Program for Changjiang Scholars and Innovative Research Team in University[IRT15R11]
项目资助者	Natural Science Foundation of China ; Natural Science Foundation of Fujian Province of China ; Shanghai Rising-Star Program ; Program for Changjiang Scholars and Innovative Research Team in University
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000531691900001
出版者	WILEY-V C H VERLAG GMBH
引用统计	被引频次：102[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/103282
专题	中国科学院合肥物质科学研究院
通讯作者	Li, Juan; Yang, Huanghao
作者单位	1.Fuzhou Univ, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Coll Chem,State Key Lab Photocatalysis Energy & E, Fuzhou 350116, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med, Hangzhou 310022, Zhejiang, Peoples R China 3.Univ Chinese Acad Sci, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 4.Fujian Med Univ, Mengchao Hepatobiliary Hosp, United Innovat Mengchao Hepatobiliary Technol Key, Fuzhou 350025, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Da,Zheng, Youshi,Lin, Ziguo,et al. Equipping Natural Killer Cells with Specific Targeting and Checkpoint Blocking Aptamers for Enhanced Adoptive Immunotherapy in Solid Tumors[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2020.
APA	Zhang, Da.,Zheng, Youshi.,Lin, Ziguo.,Liu, Xiaolong.,Li, Juan.,...&Tan, Weihong.(2020).Equipping Natural Killer Cells with Specific Targeting and Checkpoint Blocking Aptamers for Enhanced Adoptive Immunotherapy in Solid Tumors.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.
MLA	Zhang, Da,et al."Equipping Natural Killer Cells with Specific Targeting and Checkpoint Blocking Aptamers for Enhanced Adoptive Immunotherapy in Solid Tumors".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020).