Cold atmospheric plasma induces GSDME-dependent pyroptotic signaling pathway via ROS generation in tumor cells

doi:10.1038/s41419-020-2459-3

HFCAS OpenIR

	Cold atmospheric plasma induces GSDME-dependent pyroptotic signaling pathway via ROS generation in tumor cells
其他题名	Cold atmospheric plasma induces GSDME-dependent pyroptotic signaling pathway via ROS generation in tumor cells
	Yang, Xiaorui 1,2; Chen, Guodong 1; Yu, Kwan Ngok 3,4; Yang, Miaomiao 1,2,5; Peng, Shengjie 1,2; Ma, Jie 1; Qin, Feng 1,2; Cao, Wei 1,2; Cui, Shujun 1,2; Nie, Lili1 ; Han, Wei1,6,7
	2020-04-27
发表期刊	CELL DEATH & DISEASE
ISSN	2041-4889
通讯作者	Han, Wei(hanw@hfcas.ac.cn)
摘要	Cold atmospheric plasma (CAP) has been proposed as a novel promising anti-cancer treatment modality. Apoptosis and necrosis have been revealed in CAP-induced cell death, but whether CAP induces pyroptosis, another kind of programmed cell death is still unknown. In the present study, we first reported that CAP effectively induced pyroptosis in a dose-dependent manner in Gasdermin E (GSDME) high-expressed tumor cell lines. Interestingly, the basal level of GSDME protein was positively correlated with the sensitivity to CAP in three selected cancer cell lines, implying GSDME might be a potential biomarker of prognosis in the forthcoming cancer CAP treatment. Moreover, our study revealed that CAP-induced pyroptosis depended on the activation of mitochondrial pathways (JNK/cytochrome c/caspase-9/caspase-3) and the cleavage of GSDME but not Gasdermin D (GSDMD). ROS generation induced by CAP was identified to initiate the pyroptotic signaling. These results complemented our knowledge on CAP-induced cell death and provide a strategy to optimize the effect of CAP cancer treatment.
其他摘要	Cold atmospheric plasma (CAP) has been proposed as a novel promising anti-cancer treatment modality. Apoptosis and necrosis have been revealed in CAP-induced cell death, but whether CAP induces pyroptosis, another kind of programmed cell death is still unknown. In the present study, we first reported that CAP effectively induced pyroptosis in a dose-dependent manner in Gasdermin E (GSDME) high-expressed tumor cell lines. Interestingly, the basal level of GSDME protein was positively correlated with the sensitivity to CAP in three selected cancer cell lines, implying GSDME might be a potential biomarker of prognosis in the forthcoming cancer CAP treatment. Moreover, our study revealed that CAP-induced pyroptosis depended on the activation of mitochondrial pathways (JNK
DOI	10.1038/s41419-020-2459-3
关键词[WOS]	NONTHERMAL PLASMA ; HEARING-LOSS ; DFNA5 GENE ; CANCER ; APOPTOSIS ; CISPLATIN ; CASPASE-3 ; CLEAVAGE
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2019YFC 0119000] ; Chinese National Natural Science Foundation[U1632145] ; Chinese National Natural Science Foundation[81227902] ; Chinese National Natural Science Foundation[81703168] ; Natural Science Fund of Anhui Province[1608085QH181] ; CASHIPS Director's Fund[YZJJ2018QN19] ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong[IRF/0024]
项目资助者	National Key R&D Program of China ; Chinese National Natural Science Foundation ; Natural Science Fund of Anhui Province ; CASHIPS Director's Fund ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000530485500009
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：54[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/103436
专题	中国科学院合肥物质科学研究院
通讯作者	Han, Wei
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Ctr Med Phys & Technol, Anhui Prov Key Lab Med Phys & Technol, Hefei, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei, Anhui, Peoples R China 3.City Univ Hong Kong, Dept Phys, Kowloon Tong, Tat Chee Ave, Hong Kong, Peoples R China 4.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon Tong, Tat Chee Ave, Hong Kong, Peoples R China 5.Anhui Med Univ, Clin pathol Ctr, Affiliated Hosp 4, Hefei, Anhui, Peoples R China 6.Soochow Univ, Collaborat Innovat Ctr Radiat Med Jiangsu Higher, Suzhou, Jiangsu, Peoples R China 7.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Yang, Xiaorui,Chen, Guodong,Yu, Kwan Ngok,et al. Cold atmospheric plasma induces GSDME-dependent pyroptotic signaling pathway via ROS generation in tumor cells[J]. CELL DEATH & DISEASE,2020,11.
APA	Yang, Xiaorui.,Chen, Guodong.,Yu, Kwan Ngok.,Yang, Miaomiao.,Peng, Shengjie.,...&Han, Wei.(2020).Cold atmospheric plasma induces GSDME-dependent pyroptotic signaling pathway via ROS generation in tumor cells.CELL DEATH & DISEASE,11.
MLA	Yang, Xiaorui,et al."Cold atmospheric plasma induces GSDME-dependent pyroptotic signaling pathway via ROS generation in tumor cells".CELL DEATH & DISEASE 11(2020).