Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ

doi:10.1016/j.carbpol.2020.115955

HFCAS OpenIR

	Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ
	Ni, Wenfeng 1,2; Zheng, Zhiming1 ; Liu, Hui 1; Wang, Peng 1; Wang, Li 1; Wang, Han 1,2; Sun, Xiaowen 1,2; Yang, Qiang 1,2; Tang, Hengfang 1,2; Zhao, Genhai1
	2020-05-01
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
通讯作者	Zheng, Zhiming(zhengzhiming2014@163.com) ; Zhao, Genhai(zhgh327@126.com)
摘要	Prenyltransferase NovQ immobilized carboxymethyl cellulose magnetic nanoparticles (NCMNs) were successfully synthesized via a valuable approach integrated from nanocomposite preparation, and applied for the production of vitamin K-2 using menadione hydroquinol and dimethylallyl diphosphate (DMAPP) as substrates. To investigate the interaction between nanoparticles and NovQ, we characterized the nanocomposite, and revealed that carboxymethyl cellulose (CMC) and Fe3O4 formed a core-shell structure to absorb NovQ in the reaction systems, resulting from the high affinity of immobilized materials. Meanwhile, NCMNs with excellent pH and temperature tolerance, enhanced prenylated activity, and improved stability were found. Molecular docking analysis was also conducted to justify the contribution of multiple amino acids and effect of nanoparticles on catalytic properties of NovQ. Taken together, our study introduces a promising strategy to prepare magnetic nanoparticles and improve the performance of catalyst, which aims for opening new orientations for synthesis of magnetic nanoparticles used for prenyltransferase immobilization.
关键词	Carboxymethyl cellulose Magnetic nanoparticles Prenyltransferase NovQ Immobilization Prenylated activity
DOI	10.1016/j.carbpol.2020.115955
关键词[WOS]	FE3O4 NANOPARTICLES ; LIPASE ; PURIFICATION ; BIOCATALYST ; ENZYMES
收录类别	SCI
语种	英语
资助项目	Key research and development plan of Anhui Province[1804b06020342] ; Natural Science Foundation of Anhui Province[1908085MB48] ; Natural Science Foundation of Anhui Province[1908085MB43] ; China National Key Research and Development Program[2019YFA0904300] ; Major Projects of Science and Technology in Anhui Province[17030801036]
项目资助者	Key research and development plan of Anhui Province ; Natural Science Foundation of Anhui Province ; China National Key Research and Development Program ; Major Projects of Science and Technology in Anhui Province
WOS研究方向	Chemistry ; Polymer Science
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000519044900022
出版者	ELSEVIER SCI LTD
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/103646
专题	中国科学院合肥物质科学研究院
通讯作者	Zheng, Zhiming; Zhao, Genhai
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Comprehens Lab Bldg,350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Ni, Wenfeng,Zheng, Zhiming,Liu, Hui,et al. Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ[J]. CARBOHYDRATE POLYMERS,2020,235.
APA	Ni, Wenfeng.,Zheng, Zhiming.,Liu, Hui.,Wang, Peng.,Wang, Li.,...&Zhao, Genhai.(2020).Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ.CARBOHYDRATE POLYMERS,235.
MLA	Ni, Wenfeng,et al."Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ".CARBOHYDRATE POLYMERS 235(2020).