Knowledge Management System of Hefei Institute of Physical Science,CAS
Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ | |
Ni, Wenfeng1,2; Zheng, Zhiming1; Liu, Hui1; Wang, Peng1; Wang, Li1; Wang, Han1,2; Sun, Xiaowen1,2; Yang, Qiang1,2; Tang, Hengfang1,2; Zhao, Genhai1 | |
2020-05-01 | |
发表期刊 | CARBOHYDRATE POLYMERS |
ISSN | 0144-8617 |
通讯作者 | Zheng, Zhiming(zhengzhiming2014@163.com) ; Zhao, Genhai(zhgh327@126.com) |
摘要 | Prenyltransferase NovQ immobilized carboxymethyl cellulose magnetic nanoparticles (NCMNs) were successfully synthesized via a valuable approach integrated from nanocomposite preparation, and applied for the production of vitamin K-2 using menadione hydroquinol and dimethylallyl diphosphate (DMAPP) as substrates. To investigate the interaction between nanoparticles and NovQ, we characterized the nanocomposite, and revealed that carboxymethyl cellulose (CMC) and Fe3O4 formed a core-shell structure to absorb NovQ in the reaction systems, resulting from the high affinity of immobilized materials. Meanwhile, NCMNs with excellent pH and temperature tolerance, enhanced prenylated activity, and improved stability were found. Molecular docking analysis was also conducted to justify the contribution of multiple amino acids and effect of nanoparticles on catalytic properties of NovQ. Taken together, our study introduces a promising strategy to prepare magnetic nanoparticles and improve the performance of catalyst, which aims for opening new orientations for synthesis of magnetic nanoparticles used for prenyltransferase immobilization. |
关键词 | Carboxymethyl cellulose Magnetic nanoparticles Prenyltransferase NovQ Immobilization Prenylated activity |
DOI | 10.1016/j.carbpol.2020.115955 |
关键词[WOS] | FE3O4 NANOPARTICLES ; LIPASE ; PURIFICATION ; BIOCATALYST ; ENZYMES |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Key research and development plan of Anhui Province[1804b06020342] ; Natural Science Foundation of Anhui Province[1908085MB48] ; Natural Science Foundation of Anhui Province[1908085MB43] ; China National Key Research and Development Program[2019YFA0904300] ; Major Projects of Science and Technology in Anhui Province[17030801036] |
项目资助者 | Key research and development plan of Anhui Province ; Natural Science Foundation of Anhui Province ; China National Key Research and Development Program ; Major Projects of Science and Technology in Anhui Province |
WOS研究方向 | Chemistry ; Polymer Science |
WOS类目 | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
WOS记录号 | WOS:000519044900022 |
出版者 | ELSEVIER SCI LTD |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/103646 |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Zheng, Zhiming; Zhao, Genhai |
作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Comprehens Lab Bldg,350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Ni, Wenfeng,Zheng, Zhiming,Liu, Hui,et al. Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ[J]. CARBOHYDRATE POLYMERS,2020,235. |
APA | Ni, Wenfeng.,Zheng, Zhiming.,Liu, Hui.,Wang, Peng.,Wang, Li.,...&Zhao, Genhai.(2020).Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ.CARBOHYDRATE POLYMERS,235. |
MLA | Ni, Wenfeng,et al."Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ".CARBOHYDRATE POLYMERS 235(2020). |
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