MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer

doi:10.21037/tcr.2020.02.02

HFCAS OpenIR

	MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer
	Chen, Yongyi 1,2,3; Gong, Wangang 1,2,3; Dai, Wumin 1,2,3; Pan, Zhiwen 1,2,3; Xu, Xiaohong 1,2,3; Jiang, Huifen 1,2,3
	2020-02-01
发表期刊	TRANSLATIONAL CANCER RESEARCH
ISSN	2218-676X
通讯作者	Jiang, Huifen(jianghuifentougao@163.com)
摘要	Background: Gastric cancer (GC) is one of the most commonly diagnosed malignancies of the human digestive tract, and currently there is a dearth of effective biomarkers for this disease. Methods: MiR-598 expression levels were analyzed by the cancer genome atlas (TCGA database) mining and verified in GC patient plasma using real-time reverse transcription polymerase chain reaction (RTPCR) assay. We used the GEPIA and UALCAN databases and immunohistochemistry (IHC) to analyze SOX4 expression. The MTT assay assessed MNK28 and SGC7901 cell proliferation after transfection with miR-596 plasmids. The analytical tools, Functional Enrichment Analysis Tool (FunRich), Database of Immune Cell Expression (DICE) and Tumor IMmune Estimation Resource (TIMER) were used to analyze correlations between SOX4 and immune responses. Furthermore, a Kaplan Meier plotter database explored correlations between miR-596, SOX4 and overall patient survival. Results: Data from TCGA and RT-PCR indicated that miR-598 was lowly expressed in GC patients. The miRWalk database showed that SOX4 was the target genes of miR-596 and also revealed that miR-596 bound directly to SOX4. MiR-596 over-expression further depressed GC cell proliferation. In addition, the FunRich database showed that SOX4 was involved in immune responses, and was further shown to be differentially expressed in CD4+ T cells by DICE. Specifically, TIMER indicated that high expression of SOX4 was negatively correlated with infiltrating CD4+ T cells in stomach adenocarcinoma (STAD). Moreover, high expression of miR-596 and low expression of SOX4 prolonged the overall survival (OS) of GC patients. Conclusions: Our study reveals a crucial role for miR-596 in tumor-associated immune infiltration and predicting prognoses in GC patients.
关键词	Bioinformatics analysis gastric cancer (GC) immune infiltration miR-596 SOX4
DOI	10.21037/tcr.2020.02.02
关键词[WOS]	MICRORNAS ; INVASION
收录类别	SCI
语种	英语
资助项目	Zhejiang Province Public Welfare Technology Application Research Project[2018KY294]
项目资助者	Zhejiang Province Public Welfare Technology Application Research Project
WOS研究方向	Oncology
WOS类目	Oncology
WOS记录号	WOS:000518406300094
出版者	AME PUBL CO
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/103786
专题	中国科学院合肥物质科学研究院
通讯作者	Jiang, Huifen
作者单位	1.Chinese Acad Sci, Inst Canc Res & Basic Med Sci, Hangzhou 310022, Peoples R China 2.Univ Chinese Acad Sci, Canc Hosp, Dept Clin Lab, Hangzhou 310022, Peoples R China 3.Zhejiang Canc Hosp, Dept Clin Lab, 1 East Banshan Rd, Hangzhou 310022, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yongyi,Gong, Wangang,Dai, Wumin,et al. MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer[J]. TRANSLATIONAL CANCER RESEARCH,2020,9.
APA	Chen, Yongyi,Gong, Wangang,Dai, Wumin,Pan, Zhiwen,Xu, Xiaohong,&Jiang, Huifen.(2020).MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer.TRANSLATIONAL CANCER RESEARCH,9.
MLA	Chen, Yongyi,et al."MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer".TRANSLATIONAL CANCER RESEARCH 9(2020).