Improving Tumor Accumulation of Aptamers by Prolonged Blood Circulation

doi:10.1021/acs.analchem.9b05878

HFCAS OpenIR

	Improving Tumor Accumulation of Aptamers by Prolonged Blood Circulation
	Ding, Ding 1,2; Yang, Cai 1,2; Lv, Cheng 1,2; Li, Juan 1,2,3,4,5; Tan, Weihong 1,2,6,7,8
	2020-03-03
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
通讯作者	Li, Juan(lijuan@fzu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn)
摘要	Developing cancer targeted medicine depends on increasing delivery efficiency and tumor site accumulation of theranostic agents. To accomplish this, we report a modification of PTK7 receptor-specific aptamer Sgc8 with the small molecule Evans Blue (EB), thus implementing an albumin binding hitchhike strategy for prolonged blood circulation. The EB molecule could insert into the hydrophobic region of serum albumin and form an aptamer/albumin complex. This complex showed superior physiological stability, facilitating longer blood half-life, and maintaining its targeting capacity. Successful conjugation of EB-aptamers was confirmed by a series of characterization methods. Targeting performance was tested on a xenografted mouse tumor model. Taking advantage of the long circulating aptamer/HSA complex, improved accumulation, and delivery efficiency to the tumor site were achieved. Through ex vivo quantification of the EB-Sgc8 aptamers' biodistribution, the mechanism of improved targeting performance was illuminated. Therefore, the increased aptamers tumor delivery efficiency and accumulation indicate that prolonging blood circulation is a promising strategy to improve aptamers' targeted delivery performance in the future clinical translation.
DOI	10.1021/acs.analchem.9b05878
关键词[WOS]	ALBUMIN ; BINDING
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21827811] ; Shanghai Rising-Star Program[19QA1405400]
项目资助者	National Natural Science Foundation of China ; Shanghai Rising-Star Program
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000518234700080
出版者	AMER CHEMICAL SOC
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/103888
专题	中国科学院合肥物质科学研究院
通讯作者	Li, Juan; Tan, Weihong
作者单位	1.Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, IMM, Renji Hosp,Sch Med, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China 3.Fuzhou Univ, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, State Key Lab Photocatalysis Energy & Environm,Co, Fuzhou 350108, Fujian, Peoples R China 4.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China 5.Univ Chinese Acad Sci, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 6.Hunan Univ, Coll Chem & Chem Engn, Mol Sci & Biomed Lab MBL, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China 7.Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China 8.Fdn Appl Mol Evolut, Alachua, FL 32615 USA
推荐引用方式 GB/T 7714	Ding, Ding,Yang, Cai,Lv, Cheng,et al. Improving Tumor Accumulation of Aptamers by Prolonged Blood Circulation[J]. ANALYTICAL CHEMISTRY,2020,92.
APA	Ding, Ding,Yang, Cai,Lv, Cheng,Li, Juan,&Tan, Weihong.(2020).Improving Tumor Accumulation of Aptamers by Prolonged Blood Circulation.ANALYTICAL CHEMISTRY,92.
MLA	Ding, Ding,et al."Improving Tumor Accumulation of Aptamers by Prolonged Blood Circulation".ANALYTICAL CHEMISTRY 92(2020).