Overexpression of an AP2/ERF family gene, BpERF13, in birch enhances cold tolerance through upregulating CBF genes and mitigating reactive oxygen species

doi:10.1016/j.plantsci.2019.110375

HFCAS OpenIR

	Overexpression of an AP2/ERF family gene, BpERF13, in birch enhances cold tolerance through upregulating CBF genes and mitigating reactive oxygen species
	Lv, Kaiwen 1; Li, Jiang 2; Zhao, Kai 1; Chen, Su 1; Nie, Jeff 3; Zhang, Wenli 4; Liu, Guifeng 1; Wei, Hairong 5
	2020-03-01
发表期刊	PLANT SCIENCE
ISSN	0168-9452
通讯作者	Liu, Guifeng(liuguifeng@126.com) ; Wei, Hairong(hairong@mtu.edu)
摘要	The AP2/ERF (APETALA2/ethylene-responsive factor) family of transcription factors (TF) is involved in regulating biotic and abiotic stress responses in plants. To explore the role of AP2/ERFs in cold tolerance in woody plants, BpERF13 was cloned and characterized in Betula platyphylla (white birch), a species primarily found in Asia in temperate and boreal climates. Based on phylogenetic analysis, BpERF13 is a member of the IXb subfamily of ERFs. Using qRT-PCR, we found that BpERF13 was differentially expressed in different tissues, and its expression could be induced by cold treatment (4 degrees C). BpERF13 protein, fused with GFP, was exclusively localized to nuclei. To further assess the role of BpERF13 in cold tolerance, BpERF13 overexpression (OE) transgenic lines were generated in B. platyphylla and used for cold stress treatment and biochemical/physiological studies. BpERF13 overexpression lines had significantly increased tolerance to subfreezing treatment and reduced reactive oxygen species. Using a TF-centered yeast one-hybrid (Y1H) experimental system, we showed that BpERF13 could bind to LTRECOREATCOR15 and MYBCORE cis-elements to activate a reporter gene. ChIP-seq and ChIP-PCR experiments further demonstrated that BpERF13 bound to these cis-elements when present in the 5' proximal regions of superoxide dismutase (SOD), peroxidase (POD), and C-repeat-binding factor (CBF) genes. qRT-PCR was employed to examine the expression levels of these genes in response to cold stress; SOD, POD, and CBF genes were significantly upregulated in BpERF13 transgenic lines compared to wild-type plants in response to cold stress. These results indicate that the transcription factor BpERF13 regulates physiological processes underlying cold tolerance in woody plants.
关键词	Betula platyphylla ERF transcription factor Cold stress Transgenic lines ChIP-seq ChIP-PCR
DOI	10.1016/j.plantsci.2019.110375
关键词[WOS]	FREEZING TOLERANCE ; TRANSCRIPTION FACTOR ; SUPEROXIDE-DISMUTASE ; STRESS TOLERANCE ; ABSCISIC-ACID ; ARABIDOPSIS ; ACCLIMATION ; ETHYLENE ; EXPRESSION ; INVOLVEMENT
收录类别	SCI
语种	英语
资助项目	Northeast Forestry University ; National Natural Science Foundation of China[31870659]
项目资助者	Northeast Forestry University ; National Natural Science Foundation of China
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000514757000024
出版者	ELSEVIER IRELAND LTD
引用统计	被引频次：60[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/104006
专题	中国科学院合肥物质科学研究院
通讯作者	Liu, Guifeng; Wei, Hairong
作者单位	1.Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China 2.Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Ctr Med Phys & Technol, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 4.Nanjing Agr Univ, JiangSu Collaborat Innovat Ctr Modern Crop Prod, State Key Lab Crop Genet & Germplasm Enhancement, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China 5.Michigan Technol Univ, Coll Forest Resources & Environm Sci, Houghton, MI 49931 USA
推荐引用方式 GB/T 7714	Lv, Kaiwen,Li, Jiang,Zhao, Kai,et al. Overexpression of an AP2/ERF family gene, BpERF13, in birch enhances cold tolerance through upregulating CBF genes and mitigating reactive oxygen species[J]. PLANT SCIENCE,2020,292.
APA	Lv, Kaiwen.,Li, Jiang.,Zhao, Kai.,Chen, Su.,Nie, Jeff.,...&Wei, Hairong.(2020).Overexpression of an AP2/ERF family gene, BpERF13, in birch enhances cold tolerance through upregulating CBF genes and mitigating reactive oxygen species.PLANT SCIENCE,292.
MLA	Lv, Kaiwen,et al."Overexpression of an AP2/ERF family gene, BpERF13, in birch enhances cold tolerance through upregulating CBF genes and mitigating reactive oxygen species".PLANT SCIENCE 292(2020).