Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins

doi:10.1016/j.bbrc.2020.07.039

HFCAS OpenIR

	Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins
	Li, Mengqing 1,2; Jia, Jia 3,4; Zhang, Xuecheng 1,2; Dai, Haiming 3,4
	2020-09-10
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
通讯作者	Zhang, Xuecheng(turenzh@ahu.edu.cn) ; Dai, Haiming(daih@cmpt.ac.cn)
摘要	Mitophagy regulates the metabolic level and cell fates by specifically degrading damaged or redundant mitochondria in these cells. During the formation of autophagosomes, autophagy receptors and adaptors, which usually contain a LC3-interacting region (LIR) domain, are recruited through their interactions with LC3/GABARAP family of proteins. Bcl-rambo is one of the mitophagy receptors that interact with LC3s/GABARAPs. In this study, we first measured the binding of Bcl-rambo to LC3s/GABARAPs in vitro and found Bcl-rambo has a selectivity to LC3C/GABARP/GABARAPL1. Further investigations with bioinfor-matics analyses and mutagenesis suggested that the interactions with the HP1 and HP2 sites of LC3s/GABARAPs and the residues at the X-2 site of the LIR domain of Bcl-rambo are both critical for the selectivity. Moreover, assays in vivo showed that manipulating the selective binding of Bcl-rambo resulted in the changes of mitophagy inductions in the cells. Overall, our data revealed the selective binding between Bcl-rambo and LC3s/GABARAPs and its molecular mechanisms and biological signifi-cances, which will be helpful for future studies of mitophagy mediated by Bcl-rambo. (C) 2020 Elsevier Inc. All rights reserved.
关键词	Bcl-rambo LC3s/GABARAPs Mitophagy Selective binding
DOI	10.1016/j.bbrc.2020.07.039
关键词[WOS]	STRUCTURAL BASIS ; LIR MOTIF ; RECOGNITION ; DEGRADATION ; AUTOPHAGY ; ATG8/LC3
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21703254] ; National Natural Science Foundation of China[21772201] ; Second Affiliated Hospital of Anhui Medical University ; Center of Medical Physics and Technology[LHJJ202006] ; Center of Medical Physics and Technology[LHJJ202007]
项目资助者	National Natural Science Foundation of China ; Second Affiliated Hospital of Anhui Medical University ; Center of Medical Physics and Technology
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000567807200021
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/104049
专题	中国科学院合肥物质科学研究院
通讯作者	Zhang, Xuecheng; Dai, Haiming
作者单位	1.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 2.Anhui Prov Engn Technol Res Ctr Microorganisms &, Hefei 230601, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Anhui, Peoples R China 4.Chinese Acad Sci, Ctr Med Phys & Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Anhui, Peoples R China
通讯作者单位	医学物理与技术中心
推荐引用方式 GB/T 7714	Li, Mengqing,Jia, Jia,Zhang, Xuecheng,et al. Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2020,530.
APA	Li, Mengqing,Jia, Jia,Zhang, Xuecheng,&Dai, Haiming.(2020).Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,530.
MLA	Li, Mengqing,et al."Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 530(2020).