Knowledge Management System of Hefei Institute of Physical Science,CAS
Mutagenicity of 7-ketocholesterol in CHO cells: The role of lipid peroxidation | |
Wang, Xiaofei1; Li, Yintao1,2; Xia, Xuanyi1; Zhang, Min1; Ge, Chunmei1; Xia, Xiaoxiao1; Xiao, Hourong1; Xu, Shengmin3 | |
2020-12-15 | |
发表期刊 | TOXICOLOGY |
ISSN | 0300-483X |
通讯作者 | Xiao, Hourong(xhr@hfuu.edu.cn) ; Xu, Shengmin(shmxu@mail.ustc.edu.cn) |
摘要 | As an important cholesterol oxide, 7-ketocholesterol plays a deleterious role in the occurrence of cancer. Although the fact had been proved that 7-ketocholesterol could induce several biological phenomena, including apoptosis, DNA damage, et al., this issue whether 7-ketocholesterol led to mutagenesis in mammalian cells re-mains largely unexplored. Here, we investigated the major role of lipid peroxidation in the genotoxic response to 7-ketocholesterol in chinese hamster ovary (CHO) cells. The results showed that 7-ketocholesterol induced gene mutation and DNA double-strand breaks (DSBs) in concentrationand time-dependent manner. After CHO cells were treated with 25 mu M 7-ketocholesterol for 48 h, the mutation frequency at hprt gene loci and the level of gamma-H2AX protein were both significantly increased. Exposure to 7-ketocholesterol resulted in a concentration dependent increase in the apoptotic rate and the protein expression of cleaved caspase-3 and-7 in CHO cells. Moreover, a significant increase of superoxide dismutase (SOD) activity and content of malondialdehyde (MDA) was also observed. Using a inhibitor of lipid peroxidation (butylated hydroxytoluene), it was found to remarkably inhibit the genotoxicity and MDA levels caused by 7-ketocholesterol. These findings indicated that lipid peroxidation was involved in the mutagenic process of 7-ketocholesterol in CHO cells. |
关键词 | 7-ketocholesterol Mutagenicity DNA damage Apoptosis Lipid peroxidation |
DOI | 10.1016/j.tox.2020.152587 |
关键词[WOS] | DNA-DAMAGE ; ACID ; MALONDIALDEHYDE ; OXYSTEROLS ; APOPTOSIS ; REPAIR |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Natural Science Foundation of Anhui Province[1808085QB37] ; Opening Foundation of Anhui Province of Key Laboratory of Environmental Toxicology and Pollution Control Technology[Y79AH72588] ; Talent Research Foundation of Hefei University[16-17RC03] ; Natural Science Foundation of China[31670860] ; Key Project of Research and Development of Anhui Province[1804a0802224] ; Project of Scientific and Technological Research of Anhui Province[1301032168] ; Major Project of Research and Development Foundation of Hefei University[19ZR13ZDA] |
项目资助者 | Natural Science Foundation of Anhui Province ; Opening Foundation of Anhui Province of Key Laboratory of Environmental Toxicology and Pollution Control Technology ; Talent Research Foundation of Hefei University ; Natural Science Foundation of China ; Key Project of Research and Development of Anhui Province ; Project of Scientific and Technological Research of Anhui Province ; Major Project of Research and Development Foundation of Hefei University |
WOS研究方向 | Pharmacology & Pharmacy ; Toxicology |
WOS类目 | Pharmacology & Pharmacy ; Toxicology |
WOS记录号 | WOS:000593910100005 |
出版者 | ELSEVIER IRELAND LTD |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/105422 |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Xiao, Hourong; Xu, Shengmin |
作者单位 | 1.Hefei Univ, Sch Biol Food & Environm, Hefei 230601, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Cont, Hefei 230031, Peoples R China 3.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Xiaofei,Li, Yintao,Xia, Xuanyi,et al. Mutagenicity of 7-ketocholesterol in CHO cells: The role of lipid peroxidation[J]. TOXICOLOGY,2020,446. |
APA | Wang, Xiaofei.,Li, Yintao.,Xia, Xuanyi.,Zhang, Min.,Ge, Chunmei.,...&Xu, Shengmin.(2020).Mutagenicity of 7-ketocholesterol in CHO cells: The role of lipid peroxidation.TOXICOLOGY,446. |
MLA | Wang, Xiaofei,et al."Mutagenicity of 7-ketocholesterol in CHO cells: The role of lipid peroxidation".TOXICOLOGY 446(2020). |
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