Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance

doi:10.12659/MSM.927727

HFCAS OpenIR

	Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance
	Wang, Chunlei 1,2; Wei, Xiaoyan 1,2; Shao, Guoliang 1,2
	2021-02-01
发表期刊	MEDICAL SCIENCE MONITOR
ISSN	1643-3750
通讯作者	Shao, Guoliang(shaogl@zjcc.org.cn)
摘要	Background: This study investigated a nanoparticle drug delivery system to reverse multidrug resistance (MDR) and assessed its anticancer efficacy in hepatocellular carcinoma (HCC). Material/Methods: Docosahexaenoic acid (DHA) was used as the functional excipient and doxorubicin (DOX) as the chemotherapeutic drug to synthesize DOX nanoparticles (DOX-nano). The human HCC cell line HepG2 was used for experiments. HepG2/DOX, HepG2+DOX, HepG2+DOX-nano, HepG2/DOX+DOX, and HepG2/DOX+DOX-nano groups cells were treated with DOX or DOX-nano (5 mu g/mL). Nude mice bearing a HepG2/DOX xenograft were divided into model, DOX, vector-nano, and DOX-nano groups and injected with saline, DOX reagent, vector-nano, and DOX-nano (2 mg/kg), respectively. Next, cytotoxicity, cellular uptake, cell apoptosis and migration, fluorescence imaging, TUNEL assay, and tumor inhibition effects were assessed in vitro and in vivo. Furthermore, expression of MDR-related proteins was also detected using western blotting. Results: Fluorescence imaging showed that the DOX uptake in the DOX-nano-treated group was the strongest in the HCC cells or tumors. Cell apoptosis was significantly increased in DOX-nano-treated HepG2/DOX cells and tumors, and cell migration was significantly inhibited in the DOX-nano-treated HepG2/DOX cells compared with the other groups. The tumor inhibitory rate in DOX-nano-injected tumors was also significantly higher than in other groups. The expression of breast cancer resistance protein, B-cell lymphoma 2, lung resistance protein, multidrug resistance protein, and protein kinase C alpha was significantly decreased in DOX-nano-treated HepG2/DOX cells and xenograft tumors. Significantly better antitumor and MDR-reversing effects were also observed in the HepG2+DOX group compared with the HepG2/DOX group. Conclusions: This study revealed the potential efficacy of a DOX-nano drug delivery system for the treatment of HCC, using HepG2/DOX cells and nude mice bearing HepG2/DOX xenografts.
关键词	Carcinoma, Hepatocellular Docosahexaenoic Acids Doxorubicin Nanoparticles Tuberculosis, Multidrug-Resistant
DOI	10.12659/MSM.927727
关键词[WOS]	CO-DELIVERY ; CHEMOTHERAPY ; NANOCARRIERS ; TUMORS ; DRUGS ; CELLS ; DHA
收录类别	SCI
语种	英语
资助项目	Medical Health Science and Technology Project of Zhejiang Provincial Health Commission, China[2018KY287]
项目资助者	Medical Health Science and Technology Project of Zhejiang Provincial Health Commission, China
WOS研究方向	Research & Experimental Medicine
WOS类目	Medicine, Research & Experimental
WOS记录号	WOS:000613544700001
出版者	INT SCIENTIFIC INFORMATION, INC
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/119699
专题	中国科学院合肥物质科学研究院
通讯作者	Shao, Guoliang
作者单位	1.Univ Chinese Acad Sci, Pharmaceut Preparat Sect, Canc Hosp, Zhejiang Canc Hosp, Hangzhou, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chunlei,Wei, Xiaoyan,Shao, Guoliang. Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance[J]. MEDICAL SCIENCE MONITOR,2021,27.
APA	Wang, Chunlei,Wei, Xiaoyan,&Shao, Guoliang.(2021).Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance.MEDICAL SCIENCE MONITOR,27.
MLA	Wang, Chunlei,et al."Functional Doxorubicin-Loaded Omega-3 Unsaturated Fatty Acids Nanoparticles in Reversing Hepatocellular Carcinoma Multidrug Resistance".MEDICAL SCIENCE MONITOR 27(2021).