Knowledge Management System of Hefei Institute of Physical Science,CAS
Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR | |
Yu, Lu1; Fang, Wei2,3; He, Yao2,3; Cai, Wenguang4; Wei, Wei5; Tian, Changlin1,2,3 | |
2021-02-01 | |
发表期刊 | BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES |
ISSN | 0005-2736 |
通讯作者 | Yu, Lu(luyu@hmfl.ac.cn) ; Tian, Changlin(cltian@ustc.edu.cn) |
摘要 | Protein EccE(1) is an essential component of the mycobacterial ESX-1 secretion system, which plays a crucial part in the process of virulence factors secretion, especially for pathogenic mycobacteria such as Mycobacterium tuberculosis. While EccE(1) was previously postulated to be the inner membrane pore-forming unit of a membrane complex through which substrates are transported, the structural properties of EccE(1) remains to be explored. In the present study, systematic Site-Directed Spin Labeling (SDSL) and Electron Paramagnetic Resonance (EPR) spectroscopic studies was carried out to reveal the secondary structure and transmembrane topology of the Nterminal Domain of EccE(1) protein (EccE(1)-NTD) from M. smegmatis in detergent micelles. EPR-based mobility and accessibility analysis of the R1 side chain for 64 residue positions of EccE(1) -NTD indicates that the transmembrane domain adopts two a-helices spanning Phe7-Cys30 and Leu36-Ile54. A tentative structural topology model of EccE(1) -NTD embedded in membrane is also suggested based on EPR spectroscopic data in this study, which will provide further insights into this protein and the ESX secretion systems of mycobacteria. |
关键词 | Mycobacterial ESX-1 secretion system Membrane protein EccE(1) Site-directed spin labeling Electron Paramagnetic Resonance Secondary structure Transmembrane topology |
DOI | 10.1016/j.bbamem.2020.183515 |
关键词[WOS] | VII SECRETION ; ESX-1 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Natural Science Foundation of China[21825703] ; Natural Science Foundation of China[U1732275] ; Natural Science Foundation of China[21927814] ; Natural Science Foundation of China[U1832181] ; Natural Science Foundation of China[31500611] ; National Key Research and Development Program of China[2019YFA0405600] ; Users with Excellence Program of Hefei Science Center, CAS[2018HSC-UE013] ; Users with Excellence Program of Hefei Science Center, CAS[2019HSC-UE005] ; Users with Excellence Program of Hefei Science Center, CAS[2019HSC-UE020] ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology[2018ZYFX003] |
项目资助者 | Natural Science Foundation of China ; National Key Research and Development Program of China ; Users with Excellence Program of Hefei Science Center, CAS ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
WOS记录号 | WOS:000603419900018 |
出版者 | ELSEVIER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/119957 |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Yu, Lu; Tian, Changlin |
作者单位 | 1.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 4.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China 5.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China |
推荐引用方式 GB/T 7714 | Yu, Lu,Fang, Wei,He, Yao,et al. Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2021,1863. |
APA | Yu, Lu,Fang, Wei,He, Yao,Cai, Wenguang,Wei, Wei,&Tian, Changlin.(2021).Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR.BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1863. |
MLA | Yu, Lu,et al."Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE(1) from M. smegmatis using site-directed spin labeling EPR".BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1863(2021). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论