Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota

doi:10.1016/j.ecoenv.2021.111903

HFCAS OpenIR

	Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota
	Liu, Ying1,2,3 ; Wang, Tong 1,2,3; Si, Bo 1,2; Du, Hua1,2 ; Liu, Yun1,2 ; Waqas, Ahmed 1,2; Huang, Shengwei1,2 ; Zhao, Guoping1,2 ; Chen, Shaopeng1,2 ; Xu, An1,2,4
	2021-03-01
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
通讯作者	Xu, An(anxu@ipp.ac.cu)
摘要	A diverse and large community of gut microbiota reside in the intestinal tract of various organisms and play important roles in metabolism and immune homeostasis of its host. The disorders of microbiota-host interaction have been closely associated with numerous chronic inflammatory and metabolic diseases, including inflammatory bowel disease and type 2 diabetes. The accumulating evidence has shown that fine particulate matter (PM2.5) exposure contributes to the diabetes, atherosclerosis and inflammatory bowel diseases; however, few studies have explored the impact of inhaled diesel PM2.5 on gut microbiota in vivo. In this study, C57BL/6J mice were exposed to diesel PM2.5 for 14 days via intratracheal instillation, and colon tissues and feces were harvested for microbiota analysis. Using high-throughput sequencing technology, we observed that intratracheally instillated diesel PM2.5 significantly altered the gut microbiota diversity and community. At the phylum and genus levels, principal coordinate analysis (PCoA) and principal component analysis (PCA) indicated pronounced segregation of microbiota compositions, which were further confirmed by beta diversity analysis. As the most affected phylum, Bacteroidetes was greatly diminished by diesel PM2.5. On the genus level, Escherichia, Parabacteroides, Akkermansia, and Oscillibacter were significantly elevated by diesel PM2.5 exposure. Our findings provided clear evidence that exposure to diesel PM2.5 via intratracheal instillation deteriorated the gastrointestinal (GI) tract and significantly altered the structure and composition of gut microbiota, which might subsequently contribute to the developmental abnormalities of inflammation, immunity and metabolism.
关键词	Diesel PM2.5 Intratracheal instillation Gut microbiota Structure Composition
DOI	10.1016/j.ecoenv.2021.111903
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[91743106]
项目资助者	National Natural Science Foundation of China
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:000611820900001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/120180
专题	中国科学院合肥物质科学研究院
通讯作者	Xu, An
作者单位	1.Chinese Acad Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 2.Chinese Acad Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Cont, High Field Magnet Lab, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 4.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China
第一作者单位	中科院强磁场科学中心
通讯作者单位	中科院强磁场科学中心
推荐引用方式 GB/T 7714	Liu, Ying,Wang, Tong,Si, Bo,et al. Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,210.
APA	Liu, Ying.,Wang, Tong.,Si, Bo.,Du, Hua.,Liu, Yun.,...&Xu, An.(2021).Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,210.
MLA	Liu, Ying,et al."Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 210(2021).