HFCAS OpenIR
Electrophysiological characterization of photoreceptor-like cells in human inducible pluripotent stem cell-derived retinal organoids during in vitro maturation
Li, Lingyun1,2; Zhao, Huan3; Xie, Haohuan2; Akhtar, Tasneem2; Yao, Yichuan2; Cai, Yuan1; Dong, Kai1; Gu, Yonghao1; Bao, Jin2,4; Chen, Jutao2,4,5; Zhang, Mei2,4; Zhong, Kai6,7; Xu, Weiping8,9; Xue, Tian1,2,4,5
2021-03-08
Source PublicationSTEM CELLS
ISSN1066-5099
Corresponding AuthorZhao, Huan() ; Xu, Weiping() ; Xue, Tian(xuetian@ustc.edu.cn)
AbstractRetinal organoids (ROs) derived from human inducible pluripotent stem cells (hiPSCs) exhibit considerable therapeutic potential. However, current quality control of ROs during in vitro differentiation is largely limited to the detection of molecular markers, often by immunostaining, polymerase chain reaction (PCR) assays and sequencing, often without proper functional assessments. As such, in the current study, we systemically characterized the physiological maturation of photoreceptor-like cells in hiPSC-derived ROs. By performing patch-clamp recordings from photoreceptor-like cells in ROs at distinct differentiation stages (ie, Differentiation Day [D]90, D150, and D200), we determined the electrophysiological properties of the plasma membrane and several characteristic ion channels closely associated with the physiological functions of the photoreceptors. Ionic hallmarks, such as hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and cyclic nucleotide-gated (CNG) channels, matured progressively during differentiation. After D200 in culture, these characteristic currents closely resembled those in macaque or human native photoreceptors. Furthermore, we demonstrated that the hyperpolarization-activated inward current/depolarization-activated outward current ratio (I-120/I+40), termed as the inward-outward current (IOC) ratio hereon, accurately represented the maturity of photoreceptors and could serve as a sensitive indicator of pathological state. Thus, this study provides a comprehensive dataset describing the electrophysiological maturation of photoreceptor-like cells in hiPSC-derived ROs for precise and sensitive quality control during RO differentiation.
Keyworddifferentiation hiPSC ion channel patch clamp retinal organoid
DOI10.1002/stem.3363
Indexed BySCI
Language英语
Funding ProjectAnhui Provincial Key RD Program[1804h08020252] ; National Key Basic Research Program of China[2020YFA0112200] ; National Natural Science Foundation of China[31322024] ; National Natural Science Foundation of China[31571073] ; National Natural Science Foundation of China[31601134] ; National Natural Science Foundation of China[52072360] ; National Natural Science Foundation of China[61727811] ; National Natural Science Foundation of China[61890953] ; National Natural Science Foundation of China[81400407] ; National Natural Science Foundation of China[81790644] ; National Natural Science Foundation of China[81925009] ; National Natural Science Foundation of China[91432104] ; Natural Science Foundation of Anhui Province[1408085QH159] ; Natural Science Foundation of Anhui Province[1708085QC57] ; Natural Science Foundation of Anhui Province[1808085MH289] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16020603] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02010011] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB39000000]
Funding OrganizationAnhui Provincial Key RD Program ; National Key Basic Research Program of China ; National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province ; Strategic Priority Research Program of the Chinese Academy of Sciences
WOS Research AreaCell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
WOS SubjectCell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
WOS IDWOS:000626172800001
PublisherWILEY
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/120790
Collection中国科学院合肥物质科学研究院
Corresponding AuthorZhao, Huan; Xu, Weiping; Xue, Tian
Affiliation1.Univ Sci & Technol China, Affiliated Hosp 1, Div Life Sci & Med, Ctr Eye, Hefei 230001, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, CAS Key Lab Brain Funct & Dis, Hefei, Peoples R China
3.Hefei Univ, Sch Biol Food & Environm, Hefei 230601, Anhui, Peoples R China
4.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei, Peoples R China
5.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China
6.Chinese Acad Sci, High Field Magnet Lab, Hefei, Peoples R China
7.Key Lab Anhui Prov High Field Magnet Resonance Im, Hefei, Peoples R China
8.Anhui Prov Key Lab Tumor Immunotherapy & Nutr The, Hefei, Peoples R China
9.Univ Sci & Technol China, Affiliated Hosp 1, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Li, Lingyun,Zhao, Huan,Xie, Haohuan,et al. Electrophysiological characterization of photoreceptor-like cells in human inducible pluripotent stem cell-derived retinal organoids during in vitro maturation[J]. STEM CELLS,2021.
APA Li, Lingyun.,Zhao, Huan.,Xie, Haohuan.,Akhtar, Tasneem.,Yao, Yichuan.,...&Xue, Tian.(2021).Electrophysiological characterization of photoreceptor-like cells in human inducible pluripotent stem cell-derived retinal organoids during in vitro maturation.STEM CELLS.
MLA Li, Lingyun,et al."Electrophysiological characterization of photoreceptor-like cells in human inducible pluripotent stem cell-derived retinal organoids during in vitro maturation".STEM CELLS (2021).
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