HFCAS OpenIR
NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2
Zhou, Shu1,2; Pettersson, Pontus1; Huang, Jingjing3; Brzezinski, Peter1; Pomes, Regis3; Maler, Lena1; Adelroth, Pia1
2021-03-04
Source PublicationSTRUCTURE
ISSN0969-2126
Corresponding AuthorMaler, Lena(lena.maler@dbb.su.se) ; Adelroth, Pia(pia.adelroth@dbb.su.se)
AbstractThe Saccharomyces cerevisiae respiratory supercomplex factor 2 (Rcf2) is a 224-residue protein located in the mitochondrial inner membrane where it is involved in the formation of supercomplexes composed of cytochrome bc(1) and cytochrome c oxidase. We previously demonstrated that Rcf2 forms a dimer in dodecylphosphocholine micelles, and here we report the solution NMR structure of this Rcf2 dimer. Each Rcf2 monomer has two soluble alpha helices and five putative transmembrane (TM) alpha helices, including an unexpectedly charged TM helix at the C terminus, which mediates dimer formation. The NOE contacts indicate the presence of inter-monomer salt bridges and hydrogen bonds at the dimer interface, which stabilize the Rcf2 dimer structure. Moreover, NMR chemical shift change mapping upon lipid titrations as well as molecular dynamics analysis reveal possible structural changes upon embedding Rcf2 into a native lipid environment. Our results contribute to the understanding of respiratory supercomplex formation and regulation.
DOI10.1016/j.str.2020.08.008
WOS KeywordPARTICLE MESH EWALD ; MEMBRANE-PROTEIN ; CHAIN SUPERCOMPLEXES ; SOFTWARE NEWS ; FORCE-FIELD ; SPECTROSCOPY ; ASSIGNMENT ; MODEL ; RCF2 ; C-13
Indexed BySCI
Language英语
Funding ProjectKnut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT[653706] ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children
Funding OrganizationKnut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS IDWOS:000629154700009
PublisherCELL PRESS
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/121311
Collection中国科学院合肥物质科学研究院
Corresponding AuthorMaler, Lena; Adelroth, Pia
Affiliation1.Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden
2.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Peoples R China
3.Univ Toronto, Hosp Sick Children, Dept Biochem, Mol Med, Toronto, ON M5G 0A4, Canada
Recommended Citation
GB/T 7714
Zhou, Shu,Pettersson, Pontus,Huang, Jingjing,et al. NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2[J]. STRUCTURE,2021,29.
APA Zhou, Shu.,Pettersson, Pontus.,Huang, Jingjing.,Brzezinski, Peter.,Pomes, Regis.,...&Adelroth, Pia.(2021).NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2.STRUCTURE,29.
MLA Zhou, Shu,et al."NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2".STRUCTURE 29(2021).
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