NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2

doi:10.1016/j.str.2020.08.008

HFCAS OpenIR

	NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2
	Zhou, Shu1,2 ; Pettersson, Pontus 1; Huang, Jingjing 3; Brzezinski, Peter 1; Pomes, Regis 3; Maler, Lena 1; Adelroth, Pia 1
	2021-03-04
发表期刊	STRUCTURE
ISSN	0969-2126
通讯作者	Maler, Lena(lena.maler@dbb.su.se) ; Adelroth, Pia(pia.adelroth@dbb.su.se)
摘要	The Saccharomyces cerevisiae respiratory supercomplex factor 2 (Rcf2) is a 224-residue protein located in the mitochondrial inner membrane where it is involved in the formation of supercomplexes composed of cytochrome bc(1) and cytochrome c oxidase. We previously demonstrated that Rcf2 forms a dimer in dodecylphosphocholine micelles, and here we report the solution NMR structure of this Rcf2 dimer. Each Rcf2 monomer has two soluble alpha helices and five putative transmembrane (TM) alpha helices, including an unexpectedly charged TM helix at the C terminus, which mediates dimer formation. The NOE contacts indicate the presence of inter-monomer salt bridges and hydrogen bonds at the dimer interface, which stabilize the Rcf2 dimer structure. Moreover, NMR chemical shift change mapping upon lipid titrations as well as molecular dynamics analysis reveal possible structural changes upon embedding Rcf2 into a native lipid environment. Our results contribute to the understanding of respiratory supercomplex formation and regulation.
DOI	10.1016/j.str.2020.08.008
关键词[WOS]	PARTICLE MESH EWALD ; MEMBRANE-PROTEIN ; CHAIN SUPERCOMPLEXES ; SOFTWARE NEWS ; FORCE-FIELD ; SPECTROSCOPY ; ASSIGNMENT ; MODEL ; RCF2 ; C-13
收录类别	SCI
语种	英语
资助项目	Knut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT[653706] ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children
项目资助者	Knut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号	WOS:000629154700009
出版者	CELL PRESS
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/121311
专题	中国科学院合肥物质科学研究院
通讯作者	Maler, Lena; Adelroth, Pia
作者单位	1.Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden 2.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Peoples R China 3.Univ Toronto, Hosp Sick Children, Dept Biochem, Mol Med, Toronto, ON M5G 0A4, Canada
推荐引用方式 GB/T 7714	Zhou, Shu,Pettersson, Pontus,Huang, Jingjing,et al. NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2[J]. STRUCTURE,2021,29.
APA	Zhou, Shu.,Pettersson, Pontus.,Huang, Jingjing.,Brzezinski, Peter.,Pomes, Regis.,...&Adelroth, Pia.(2021).NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2.STRUCTURE,29.
MLA	Zhou, Shu,et al."NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2".STRUCTURE 29(2021).