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| NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2 | |
Zhou, Shu1,2 ; Pettersson, Pontus1; Huang, Jingjing3; Brzezinski, Peter1; Pomes, Regis3; Maler, Lena1; Adelroth, Pia1
| |
| 2021-03-04 | |
| Source Publication | STRUCTURE
 |
| ISSN | 0969-2126 |
| Corresponding Author | Maler, Lena(lena.maler@dbb.su.se) ; Adelroth, Pia(pia.adelroth@dbb.su.se) |
| Abstract | The Saccharomyces cerevisiae respiratory supercomplex factor 2 (Rcf2) is a 224-residue protein located in the mitochondrial inner membrane where it is involved in the formation of supercomplexes composed of cytochrome bc(1) and cytochrome c oxidase. We previously demonstrated that Rcf2 forms a dimer in dodecylphosphocholine micelles, and here we report the solution NMR structure of this Rcf2 dimer. Each Rcf2 monomer has two soluble alpha helices and five putative transmembrane (TM) alpha helices, including an unexpectedly charged TM helix at the C terminus, which mediates dimer formation. The NOE contacts indicate the presence of inter-monomer salt bridges and hydrogen bonds at the dimer interface, which stabilize the Rcf2 dimer structure. Moreover, NMR chemical shift change mapping upon lipid titrations as well as molecular dynamics analysis reveal possible structural changes upon embedding Rcf2 into a native lipid environment. Our results contribute to the understanding of respiratory supercomplex formation and regulation. |
| DOI | 10.1016/j.str.2020.08.008 |
| WOS Keyword | PARTICLE MESH EWALD ; MEMBRANE-PROTEIN ; CHAIN SUPERCOMPLEXES ; SOFTWARE NEWS ; FORCE-FIELD ; SPECTROSCOPY ; ASSIGNMENT ; MODEL ; RCF2 ; C-13 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Knut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT[653706] ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children |
| Funding Organization | Knut and Alice Wallenberg Foundation ; Swedish Research Council ; iNEXT ; Horizon 2020 programme of the European Commission ; Natural Sciences and Engineering Council of Canada (NSERC) ; Hospital for Sick Children |
| WOS Research Area | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| WOS Subject | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| WOS ID | WOS:000629154700009 |
| Publisher | CELL PRESS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.hfcas.ac.cn:8080/handle/334002/121311 |
| Collection | 中国科学院合肥物质科学研究院 |
| Corresponding Author | Maler, Lena; Adelroth, Pia |
| Affiliation | 1.Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden 2.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Peoples R China 3.Univ Toronto, Hosp Sick Children, Dept Biochem, Mol Med, Toronto, ON M5G 0A4, Canada |
| Recommended Citation GB/T 7714 | Zhou, Shu,Pettersson, Pontus,Huang, Jingjing,et al. NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2[J]. STRUCTURE,2021,29. |
| APA | Zhou, Shu.,Pettersson, Pontus.,Huang, Jingjing.,Brzezinski, Peter.,Pomes, Regis.,...&Adelroth, Pia.(2021).NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2.STRUCTURE,29. |
| MLA | Zhou, Shu,et al."NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2".STRUCTURE 29(2021). |
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