A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment*

doi:10.1088/1674-1056/abda32

HFCAS OpenIR

	A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment*
	Wang, Kun-Yang 1,2,3; Shao, Jie 3; Shao, Li-Gang 1; Chen, Jia-Jin 1; Wang, Gui-Shi 1; Liu, Kun1 ; Gao, Xiao-Ming1,2
	2021-05-01
发表期刊	CHINESE PHYSICS B
ISSN	1674-1056
通讯作者	Shao, Jie(shaojie@zjnu.cn)
摘要	A new pressure-calibration method for calibrating the reduction of second harmonic (2f) amplitude caused by pressure broadening effect in sealed microbial growth environment is present. The new method combines with linewidth compensation and modulation depth compensation and makes the 2f amplitude accurately retrieve metabolic CO2 in microbial growth. In order to verify the method, a simulation experiment is developed, in which the increasing CO2 concentration leads to the increasing pressure. Comparing with the relation between the traditional 2f amplitude and gas concentration, there is a monotonous relation between the calibrated 2f amplitude and CO2 concentration, particularly, a linear relation is present when the CO2 concentration is replaced with the CO2 particle number. In terms of microbial measurement, the growth of Escherichia coli is measured, and the culture bottle is sealed during the microbial growth process. The experimental results show that, comparing to the microbial growth retrieved by traditional 2f amplitude, the calibrated 2f amplitude can accurately retrieve microbial growth in sealed environment.
关键词	second harmonic amplitude pressure-calibration microbial growth sealed environment CO2
DOI	10.1088/1674-1056/abda32
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2017YFC0209700] ; National Key Research and Development Program of China[2016YFC0303900] ; National Natural Science Foundation of China[41730103]
项目资助者	National Key Research and Development Program of China ; National Natural Science Foundation of China
WOS研究方向	Physics
WOS类目	Physics, Multidisciplinary
WOS记录号	WOS:000647651400001
出版者	IOP PUBLISHING LTD
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/121993
专题	中国科学院合肥物质科学研究院
通讯作者	Shao, Jie
作者单位	1.Chinese Acad Sci, Anhui Inst Opt & Fine Mech, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230031, Peoples R China 3.Zhejiang Normal Univ, Coll Phys & Elect Informat Engn, Jinhua 321004, Zhejiang, Peoples R China
第一作者单位	中科院安徽光学精密机械研究所
推荐引用方式 GB/T 7714	Wang, Kun-Yang,Shao, Jie,Shao, Li-Gang,et al. A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment*[J]. CHINESE PHYSICS B,2021,30.
APA	Wang, Kun-Yang.,Shao, Jie.,Shao, Li-Gang.,Chen, Jia-Jin.,Wang, Gui-Shi.,...&Gao, Xiao-Ming.(2021).A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment*.CHINESE PHYSICS B,30.
MLA	Wang, Kun-Yang,et al."A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment*".CHINESE PHYSICS B 30(2021).