Knowledge Management System of Hefei Institute of Physical Science，CAS
| Gradient radiation breeding and culture domestication of menaquinone producing strains | |
Wu, Hefang1,2; Wang, Han1; Wang, Peng1 ; Zhao, Geihai1; Liu, Hui1; Wang, Li1; Sun, Xiaowen1; Zheng, Zhiming1
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| 2021-05-18 | |
| Source Publication | BIOPROCESS AND BIOSYSTEMS ENGINEERING
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| ISSN | 1615-7591 |
| Corresponding Author | Zheng, Zhiming(zhengzhiming2014@163.com) |
| Abstract | By comparing the survival rate and positive mutation rate of the primary mutagenic strain and progeny mutagenic strain under different radiation doses, the results showed that the tolerance of the mutagenic strain to radiation dose increased with the increase of the mutagenic generations. We adopted an improved gradient radiation breeding strategy to improve the breeding efficiency. The strains were treated with radiation in four stages. The first stage was low energy N+ ion implantation (ion energy 15 keV, dose 80 x 2.6 x 10(13) cm(-2)). In the second stage, the energy and dose of N+ ion reached to 20 keV, 90 x 2.6 x 10(13) cm(-2). In the third stage, Co-60-gamma radiation (dose of 1.56 kGy) was used. In the fourth stage, the radiation dose of Co-60-gamma increased to 1.82 kGy. After each stage of radiation, the MK (Menaquinone) precursor 1, 4-dihydroxy-2-naphthalate (DHNA) was used as the stress factor to domesticate the mutant strains. By gradually increasing the concentration of DHNA in the culture medium, the substrate tolerance of Flavobacterium sp. was effectively improved. By measuring SOD (superoxide dismutase) activity and malondialdehyde, it showed that the cell damage caused by radiation mutagenesis to the offspring mutant was less than that of the primary mutant. Changes in membrane permeability and membrane potential of the mutant strains were reflected in changes in fluorescence intensity of luciferin diacetate and rhodamine 123, which could explain the enhanced substrate tolerance of strain F-2. After gradient radiation breeding and culture acclimation, the biomass of mutant Strain F-2 was 6.59 g/L, and the MK yield was 9.59 mg/L. |
| Keyword | Biofilm lipid peroxidation Gamma ray mutagenesis Low-energy ion implantation Menaquinone (MK) SOD activity |
| DOI | 10.1007/s00449-021-02508-8 |
| WOS Keyword | SUPEROXIDE-DISMUTASE ; HUMAN OSTEOBLASTS ; VITAMIN-K ; APOPTOSIS ; MUTATION ; FOOD |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Hi-Tech Research and Development Program of China (key 863 Fund of China)[2014AA021704] ; Natural Science Foundation of Anhui Province[1308085MA07] ; Natural Science Foundation of Anhui Province[1608085QC46] |
| Funding Organization | Hi-Tech Research and Development Program of China (key 863 Fund of China) ; Natural Science Foundation of Anhui Province |
| WOS Research Area | Biotechnology & Applied Microbiology ; Engineering |
| WOS Subject | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| WOS ID | WOS:000651666500001 |
| Publisher | SPRINGER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.hfcas.ac.cn:8080/handle/334002/122120 |
| Collection | 中国科学院合肥物质科学研究院 |
| Corresponding Author | Zheng, Zhiming |
| Affiliation | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Shushanhu Rd 350, Hefei 210031, Anhui, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Hefang,Wang, Han,Wang, Peng,et al. Gradient radiation breeding and culture domestication of menaquinone producing strains[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2021. |
| APA | Wu, Hefang.,Wang, Han.,Wang, Peng.,Zhao, Geihai.,Liu, Hui.,...&Zheng, Zhiming.(2021).Gradient radiation breeding and culture domestication of menaquinone producing strains.BIOPROCESS AND BIOSYSTEMS ENGINEERING. |
| MLA | Wu, Hefang,et al."Gradient radiation breeding and culture domestication of menaquinone producing strains".BIOPROCESS AND BIOSYSTEMS ENGINEERING (2021). |
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