HFCAS OpenIR
Gradient radiation breeding and culture domestication of menaquinone producing strains
Wu, Hefang1,2; Wang, Han1; Wang, Peng1; Zhao, Geihai1; Liu, Hui1; Wang, Li1; Sun, Xiaowen1; Zheng, Zhiming1
2021-05-18
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Corresponding AuthorZheng, Zhiming(zhengzhiming2014@163.com)
AbstractBy comparing the survival rate and positive mutation rate of the primary mutagenic strain and progeny mutagenic strain under different radiation doses, the results showed that the tolerance of the mutagenic strain to radiation dose increased with the increase of the mutagenic generations. We adopted an improved gradient radiation breeding strategy to improve the breeding efficiency. The strains were treated with radiation in four stages. The first stage was low energy N+ ion implantation (ion energy 15 keV, dose 80 x 2.6 x 10(13) cm(-2)). In the second stage, the energy and dose of N+ ion reached to 20 keV, 90 x 2.6 x 10(13) cm(-2). In the third stage, Co-60-gamma radiation (dose of 1.56 kGy) was used. In the fourth stage, the radiation dose of Co-60-gamma increased to 1.82 kGy. After each stage of radiation, the MK (Menaquinone) precursor 1, 4-dihydroxy-2-naphthalate (DHNA) was used as the stress factor to domesticate the mutant strains. By gradually increasing the concentration of DHNA in the culture medium, the substrate tolerance of Flavobacterium sp. was effectively improved. By measuring SOD (superoxide dismutase) activity and malondialdehyde, it showed that the cell damage caused by radiation mutagenesis to the offspring mutant was less than that of the primary mutant. Changes in membrane permeability and membrane potential of the mutant strains were reflected in changes in fluorescence intensity of luciferin diacetate and rhodamine 123, which could explain the enhanced substrate tolerance of strain F-2. After gradient radiation breeding and culture acclimation, the biomass of mutant Strain F-2 was 6.59 g/L, and the MK yield was 9.59 mg/L.
KeywordBiofilm lipid peroxidation Gamma ray mutagenesis Low-energy ion implantation Menaquinone (MK) SOD activity
DOI10.1007/s00449-021-02508-8
WOS KeywordSUPEROXIDE-DISMUTASE ; HUMAN OSTEOBLASTS ; VITAMIN-K ; APOPTOSIS ; MUTATION ; FOOD
Indexed BySCI
Language英语
Funding ProjectHi-Tech Research and Development Program of China (key 863 Fund of China)[2014AA021704] ; Natural Science Foundation of Anhui Province[1308085MA07] ; Natural Science Foundation of Anhui Province[1608085QC46]
Funding OrganizationHi-Tech Research and Development Program of China (key 863 Fund of China) ; Natural Science Foundation of Anhui Province
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000651666500001
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/122120
Collection中国科学院合肥物质科学研究院
Corresponding AuthorZheng, Zhiming
Affiliation1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Shushanhu Rd 350, Hefei 210031, Anhui, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wu, Hefang,Wang, Han,Wang, Peng,et al. Gradient radiation breeding and culture domestication of menaquinone producing strains[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2021.
APA Wu, Hefang.,Wang, Han.,Wang, Peng.,Zhao, Geihai.,Liu, Hui.,...&Zheng, Zhiming.(2021).Gradient radiation breeding and culture domestication of menaquinone producing strains.BIOPROCESS AND BIOSYSTEMS ENGINEERING.
MLA Wu, Hefang,et al."Gradient radiation breeding and culture domestication of menaquinone producing strains".BIOPROCESS AND BIOSYSTEMS ENGINEERING (2021).
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