Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis

doi:10.1007/s13577-021-00541-z

HFCAS OpenIR

	Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis
	Huang, Yuan 1,2; Zheng, Yabing 1,2; Shao, Xiying 1,2; Shi, Lei1,2 ; Li, Guangliang 1,2; Huang, Ping 1,2
	2021-05-17
发表期刊	HUMAN CELL
ISSN	0914-7470
通讯作者	Zheng, Yabing(zhengyabingcas@tom.com)
摘要	Long non-coding RNAs (lncRNAs) are key modulators during cancer progression. Application of using lncRNA expression to evaluate patient prognosis and sensitivity to treatment is highly anticipated, yet the expression and mechanism of many lncRNAs remain unknown. Herein, we projected for the investigation of TPT1-AS1 function in breast cancer. TPT1-AS1 was assessed by bioinformatic analysis of publicly available datasets and quantitative real-time PCR (qRT-PCR). Cell sensitivity to paclitaxel and cell proliferation was measured by flow cytometry and CCK-8. Interaction among TPT1-AS1, microRNA (miRNA, miR)-3156-5p and Caspase 2 (CASP2) was studied by bioinformatic analysis, qRT-PCR, western blot as well as dual luciferase reporter assay. Herein, TPT1-AS1 was significantly diminished in breast cancer from publicly available datasets and our collected samples. In breast cancer cells, TPT1-AS1 overexpression repressed cell proliferation and sensitized breast cancer cells to paclitaxel. RegRNA 2.0 predicted a potential interaction between TPT1-AS1 and miR-3156-5p which was confirmed by qRT-PCR as well as dual luciferase reporter assay. CASP2, a proapoptotic gene, was corroborated to be targeted by miR-3156-5p. Meanwhile, TPT1-AS1 upregulated CASP2 in breast cancer cells, and its biological function was reversed by CASP2 knockdown. Collectively, TPT1-AS1 diminished cell proliferation and sensitized cells to chemotherapy by sponging miR-3156-5p and upregulating CASP2, acting as a biomarker for patients with breast cancer.
关键词	TPT1-AS1 Cell proliferation Paclitaxel sensitivity Breast cancer CASP2
DOI	10.1007/s13577-021-00541-z
关键词[WOS]	GENE ; RESISTANCE ; EXPRESSION ; APOPTOSIS ; PROMOTES
收录类别	SCI
语种	英语
资助项目	Zhejiang Provincial Medical Science and Technology program[2016RCA003] ; Zhejiang Science and Technology program[2016C33199]
项目资助者	Zhejiang Provincial Medical Science and Technology program ; Zhejiang Science and Technology program
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000651333300002
出版者	SPRINGER JAPAN KK
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/122165
专题	中国科学院合肥物质科学研究院
通讯作者	Zheng, Yabing
作者单位	1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Breast Med Oncol, 1 East Banshan Rd, Hangzhou 310022, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Dept Breast Med Oncol, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yuan,Zheng, Yabing,Shao, Xiying,et al. Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis[J]. HUMAN CELL,2021.
APA	Huang, Yuan,Zheng, Yabing,Shao, Xiying,Shi, Lei,Li, Guangliang,&Huang, Ping.(2021).Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis.HUMAN CELL.
MLA	Huang, Yuan,et al."Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis".HUMAN CELL (2021).