NMR structural study on the self-trimerization of d(GTTAGG) into a dynamic trimolecular G-quadruplex assembly preferentially in Na+ solution with a moderate K+ tolerance

doi:10.1093/nar/gkab028

HFCAS OpenIR

	NMR structural study on the self-trimerization of d(GTTAGG) into a dynamic trimolecular G-quadruplex assembly preferentially in Na+ solution with a moderate K+ tolerance
	Jing, Haitao 1,2; Fu, Wenqiang 1,2; Hu, Wenxuan 1,2; Xu, Suping 1; Xu, Xiaojuan 1,2; He, Miao 1,2; Liu, Yangzhong 2; Zhang, Na1,3,4,5
	2021-02-26
发表期刊	NUCLEIC ACIDS RESEARCH
ISSN	0305-1048
通讯作者	Zhang, Na(nazhang@hmfl.cas.cn)
摘要	Vast G-quadruplexes (GQs) are primarily folded by one, two, or four G-rich oligomers, rarely with an exception. Here, we present the first NMR solution structure of a trimolecular GQ (tri-GQ) that is solely assembled by the self-trimerization of d(GTTAGG), preferentially in Na+ solution tolerant to an equal amount of K+ cation. Eight guanines from three asymmetrically folded strands of d(GTTAGG) are organized into a two-tetrad core, which features a broken G-column and two width-irregular grooves. Fast strand exchanges on a timescale of second at 17 degrees C spontaneously occur between folded tri-GQ and unfolded single-strand of d(GTTAGG) that both species coexist in dynamic equilibrium. Thus, this tri-GQ is not just simply a static assembly but rather a dynamic assembly. Moreover, another minor tetra-GQ that has putatively tetrameric (2+2) antiparallel topology becomes noticeable only at an extremely high strand concentration above 18 mM. The major tri-GQ and minor tetra-GQ are considered to be mutually related, and their reversible interconversion pathways are proposed accordingly. The sequence d(GTTAGG) could be regarded as either a reading frame shifted single repeat of human telomeric DNA or a 1.5 repeat of Bombyx mori telomeric DNA. Overall, our findings provide new insight into GQs and expect more functional applications.
DOI	10.1093/nar/gkab028
关键词[WOS]	ENZYMATIC-SYNTHESIS ; C-13,N-15-LABELED DNA ; SEQUENCE ; STABILITY ; TOPOLOGY ; REGION
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2016YFA0400900] ; National Natural Science Foundation of China[U1932157] ; National Natural Science Foundation of China[21372223] ; National Natural Science Foundation of China[U1232145] ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[U1932157] ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2018ZYFX004]
项目资助者	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000637321900038
出版者	OXFORD UNIV PRESS
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/122386
专题	中国科学院合肥物质科学研究院
通讯作者	Zhang, Na
作者单位	1.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China 4.Key Lab Anhui Prov High Field Magnet Resonance Im, Hefei 230031, Peoples R China 5.High Magnet Field Lab Anhui Prov, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Jing, Haitao,Fu, Wenqiang,Hu, Wenxuan,et al. NMR structural study on the self-trimerization of d(GTTAGG) into a dynamic trimolecular G-quadruplex assembly preferentially in Na+ solution with a moderate K+ tolerance[J]. NUCLEIC ACIDS RESEARCH,2021,49.
APA	Jing, Haitao.,Fu, Wenqiang.,Hu, Wenxuan.,Xu, Suping.,Xu, Xiaojuan.,...&Zhang, Na.(2021).NMR structural study on the self-trimerization of d(GTTAGG) into a dynamic trimolecular G-quadruplex assembly preferentially in Na+ solution with a moderate K+ tolerance.NUCLEIC ACIDS RESEARCH,49.
MLA	Jing, Haitao,et al."NMR structural study on the self-trimerization of d(GTTAGG) into a dynamic trimolecular G-quadruplex assembly preferentially in Na+ solution with a moderate K+ tolerance".NUCLEIC ACIDS RESEARCH 49(2021).