miR-221-3p and miR-222-3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer

doi:10.3892/etm.2021.10084

HFCAS OpenIR

	miR-221-3p and miR-222-3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer
	Ye, Ting 1,2; Zhong, Lili 3; Ye, Xuemei 1,2; Liu, Jie 1,2; Li, Linfa 1,2; Yi, Heqing 1,2
	2021-06-01
发表期刊	EXPERIMENTAL AND THERAPEUTIC MEDICINE
ISSN	1792-0981
通讯作者	Li, Linfa(pet-ct001@163.com) ; Yi, Heqing(yiheqing1980@163.com)
摘要	The expression levels of microRNA (miR)-221-3p and miR-222-3p in thyroid cancer have been found to be upregulated compared with those in normal tissues. The present study aimed to determine the effects and potential underlying mechanisms of miR-221-3p and miR-222-3p on the regulation of radioactive iodine (I-131) uptake and radiosensitivity of thyroid cancer cells. The potential regulatory target genes of miR-221-3p and miR-222-3p were predicted by bioinformatics analysis, and reverse transcription-quantitative polymerase chain reaction was used to verify miR-221-3p, miR-222-3p and target gene expression levels in thyroid cancer tissues and cell lines. Overexpression of miR-221-3p or miR-222-3p in cell models was performed using lentivirus infection. Knockdown of miR-221-3p and miR-222-3p in cells was achieved using oligonucleotide inhibitor transfection. Western blotting was used to analyze the expression levels of target proteins. In addition, the effects of miR-221-3p and miR-222-3p on the radiosensitivity of thyroid cancer cells were verified using a colony formation assay. The results of the present study revealed that the expression levels of miR-221-3p and miR-222-3p were significantly upregulated, while the expression levels of suppressor of cytokine signaling 3 (SOCS3) were downregulated in thyroid cancer tissues. Furthermore, miR-221-3p and miR-222-3p overexpression downregulated the expression levels of SOCS3, E-cadherin and solute carrier family 5 member 5 (NIS), and upregulated the expression levels of phosphorylated STAT3 and vimentin. Following the overexpression of miR-221-3p or miR-222-3p in the FTC133 and TPC1 cell lines, their radiosensitivity was suppressed. In conclusion, the findings of the present study suggested that miR-221-3p and miR-222-3p may downregulate the expression levels of NIS and promote radioresistance. The potential mechanism was hypothesized to be associated with the miR-221-3p and miR-222-3p targeting of the SOCS3 gene, which may subsequently activate the STAT3 signaling pathway.
关键词	differentiated thyroid cancer microRNA-221 microRNA-222 suppressor of cytokine signaling 3 solute carrier family 5 member 5 radiosensitivity
DOI	10.3892/etm.2021.10084
关键词[WOS]	CELLS
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81502318] ; Zhejiang Province Natural Science Foundation of China[LY20H180007]
项目资助者	National Natural Science Foundation of China ; Zhejiang Province Natural Science Foundation of China
WOS研究方向	Research & Experimental Medicine
WOS类目	Medicine, Research & Experimental
WOS记录号	WOS:000648662100001
出版者	SPANDIDOS PUBL LTD
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/122452
专题	中国科学院合肥物质科学研究院
通讯作者	Li, Linfa; Yi, Heqing
作者单位	1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Key Lab Head & Neck Canc Translat Res Zhejiang Pr, Dept Nucl Med,Canc Hosp, 1 Banshan East Rd, Hangzhou 310021, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou 310021, Zhejiang, Peoples R China 3.Second Hosp Jilin Univ, Jilin Prov Key Lab Mol & Chem Genet, Changchun 130022, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Ye, Ting,Zhong, Lili,Ye, Xuemei,et al. miR-221-3p and miR-222-3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2021,21.
APA	Ye, Ting,Zhong, Lili,Ye, Xuemei,Liu, Jie,Li, Linfa,&Yi, Heqing.(2021).miR-221-3p and miR-222-3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer.EXPERIMENTAL AND THERAPEUTIC MEDICINE,21.
MLA	Ye, Ting,et al."miR-221-3p and miR-222-3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer".EXPERIMENTAL AND THERAPEUTIC MEDICINE 21(2021).