Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori

doi:10.1021/acs.analchem.1c00663

HFCAS OpenIR

	Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori
	Celik, Cagla 1,2; Sezgin, Gulten Can 3; Kocabas, Umut Gorkem 1; Gursoy, Sebnem 3; Ildiz, Nilay 4; Tan, Weihong 5,6; Ocsoy, Ismail 1
	2021-04-20
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
通讯作者	Ildiz, Nilay(nilaygucluer@erciyes.edu.tr) ; Tan, Weihong(tan@hnu.edu.cn) ; Ocsoy, Ismail(ismailocsoy@erciyes.edu.tr)
摘要	Several different diagnostic tests have been reported for rapid, sensitive, and economical detection of bacterial pathogens, but most lack widespread and practical use in the clinic. In this study, we used anthocyanins from red cabbage (Brassica oleracea) as a natural pH indicator and, for the first time, incorporated this agent into a simple, rapid, and economical colorimetric strategy for the detection of Helicobacter pylori (H. pylori) (RCE@test). We prepared two sets of RCE@test solutions (test 1 is purple, and test 2 is blue) in different forms, including liquid, adsorbed filter paper, and agar, and investigated the performance of each RCE@test as a function of the test volume, H. pylori concentration, and reaction time. To elucidate the effect of the pathophysiological environment on these RCE@tests, H. pylori in an artificial gastric fluid was also detected. The 10 and 1 CFU/mL H. pylori suspensions were detected in 15 min and 3 h, respectively, and the limit of detection was determined down to 1 CFU/mL. We experimentally demonstrated the advantages of the RCE@test for detection of H. pylori by comparing it to a commercially available rapid urease test, the "CLO test (Campylobacter-like organism test)". In addition to colorimetric detection by the naked eyes, RGB (Red Green Blue) and Delta-E analysis in image-processing software was run to quantitatively monitor changes of color in the RCE@test using a smartphone application. Finally, we propose that this test provides simple, effective, rapid, and inexpensive detection and that it can be easily implemented for clinical use.
DOI	10.1021/acs.analchem.1c00663
关键词[WOS]	RED CABBAGE ; INFECTION ; DIAGNOSIS ; NANOPARTICLES ; STRATEGIES ; WORLDWIDE ; COLOR
收录类别	SCI
语种	英语
资助项目	2020 BAGEP Award of the Science Academy ; Turkish Academy of Sciences (2018 TUBA GEBIP Award)
项目资助者	2020 BAGEP Award of the Science Academy ; Turkish Academy of Sciences (2018 TUBA GEBIP Award)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000643539400033
出版者	AMER CHEMICAL SOC
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/122624
专题	中国科学院合肥物质科学研究院
通讯作者	Ildiz, Nilay; Tan, Weihong; Ocsoy, Ismail
作者单位	1.Erciyes Univ, Fac Pharm, Dept Analyt Chem, TR-38039 Kayseri, Turkey 2.Hitit Univ, Vocat Sch Hlth Serv, Pharm Serv Program, TR-19000 Corum, Turkey 3.Erciyes Univ, Fac Med, Dept Gastroenterol, TR-38039 Kayseri, Turkey 4.Erciyes Univ, Fac Pharm, Dept Pharmaceut Microbiol, TR-38039 Kayseri, Turkey 5.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Basic Med & Canc IBMC, Canc Hosp,Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 6.Hunan Univ, Coll Chem & Chem Engn, Coll Biol,State Key Lab Chemo Biosensing & Chemom, Aptamer Engn Ctr Hunan Prov,Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Celik, Cagla,Sezgin, Gulten Can,Kocabas, Umut Gorkem,et al. Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori[J]. ANALYTICAL CHEMISTRY,2021,93.
APA	Celik, Cagla.,Sezgin, Gulten Can.,Kocabas, Umut Gorkem.,Gursoy, Sebnem.,Ildiz, Nilay.,...&Ocsoy, Ismail.(2021).Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori.ANALYTICAL CHEMISTRY,93.
MLA	Celik, Cagla,et al."Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori".ANALYTICAL CHEMISTRY 93(2021).