Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging

doi:10.1016/S1872-2040(21)60108-1

HFCAS OpenIR

	Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging
	Fan Zi-Yan 1; Liu Zheng-Jie 1; Zhang Rui-Long 1; Han Guang-Mei 1; Zhang Zhong-Ping1,2
	2021-07-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
通讯作者	Han Guang-Mei(gmhan@ahu.edu.cn)
摘要	The lysosome-targeting carbon dots (CDs) with excellent water solubility and strong blue emission were synthesized through a one-step functional modification for long-term lysosome imaging in living cells. Based on the carboxyl-rich CDs synthesized by oxidizing carbon black in refluxing HNO3, then N,N-dimethylethylenediamine (DMEDA) was chemically linked onto the CDs through acylation reaction to obtain DMEDA modified carbon dots (M-CDs). The infrared spectrum of M-CDs showed the characteristic peak of acylamide (O=C-NH-) at 1658 cm(-1), indicating the successful modification of DMEDA. The quantum yield of M-CDs was 15.6%, which was 27 times higher than the initial carboxyl rich CDs, meeting the requirement of cell imaging. The probe entered cells through the temperature-dependent endocytosis way and the co-localization experiment with commercial dye Lyso-Tracker red showed that the probe localized in lysosomes and could be used as a universal lysosome tracker in different cell lines. Moreover, compared to commercially available Lyso-Tracker blue, M-CDs were more photostable under UV light. The fluorescence of M-CDs was still brightness after time-lapsed imaging by confocal microscopy for 120 min with an interval of 5 min in living cells, implying that the M-CDs could be used for long-term imaging of lysosomes.
关键词	Carbon dots Functional modification N,N-dimethylethylenediamine Lysosome target Cell imaging
DOI	10.1016/S1872-2040(21)60108-1
关键词[WOS]	GRAPHENE QUANTUM DOTS ; FLUORESCENT-PROBE ; NANOPROBES
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[22074001] ; National Natural Science Foundation of China[21874137] ; National Natural Science Foundation of China[21974001] ; National Natural Science Foundation of China[21775001] ; Science and Technology Service Network Initiative of Chinese Academy of Sciences[KFJ-STS-ZDTP-080]
项目资助者	National Natural Science Foundation of China ; Science and Technology Service Network Initiative of Chinese Academy of Sciences
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000670603700015
出版者	SCIENCE PRESS
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/123494
专题	中国科学院合肥物质科学研究院
通讯作者	Han Guang-Mei
作者单位	1.Anhui Univ, Sch Chem & Chem Engn, Inst Phys Sci & Informat Technol, Informat Mat & Intelligent Sensing Lab Anhui Prov, Hefei 230601, Peoples R China 2.Chinese Acad Sci, Inst Solid State Phys, Key Lab Photovolta & Energy Conservat Mat, HFIPS, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Fan Zi-Yan,Liu Zheng-Jie,Zhang Rui-Long,et al. Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2021,49.
APA	Fan Zi-Yan,Liu Zheng-Jie,Zhang Rui-Long,Han Guang-Mei,&Zhang Zhong-Ping.(2021).Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,49.
MLA	Fan Zi-Yan,et al."Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 49(2021).