Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis
Shao, Chang-Sheng1,2; Feng, Na3,4; Zhou, Shuai3; Zheng, Xin-Xin1,2; Wang, Peng1; Zhang, Jing-Song3,4; Huang, Qing1,2
Corresponding AuthorZhang, Jing-Song(syja16@saas.sh.cn) ; Huang, Qing(huangq@ipp.ac.cn)
AbstractThe use of natural substances derived from traditional Chinese medicine and natural plants as safe radiosensitizing adjuvants is a new trend for cancer radiotherapy. Ganoderma lucidum has been used as a traditional Chinese medicine with a history of more than 2000 years. Ganoderic acid T (GAT) is a typical triterpene of G. lucidum, which has strong cytotoxicity to cancer cells, but whether it has radiation sensitization effect has not been explored. In this work, we treated the HeLa cells with different concentrations of GAT before exposure to gamma-ray radiation and investigated its influence on the radiosensitivity. The cell viability, apoptosis rate, necoptosis rate, intracellular ATP level, cell cycle, the amount of H2AX and 53BP1, reactive oxygen species, and mitochondrial membrane potential were examined. Apoptotic, necroptotic, and autophagic biomarker proteins, including caspase 8, cytochrome c, caspase 3, RIPK, MLKL, P62, and LC3, were analyzed. As a result, we confirmed that with treatment of GAT, the gamma-ray radiation induced both apoptosis and necroptosis in HeLa cells, and with increase of GAT, the percentage ratio of necroptosis was increased. The involved pathways and mechanisms were also explored and discussed.
Keywordganoderic acid T (GAT) triterpene apoptosis necroptosis radiosensitivity ionizing radiation
Indexed BySCI
Funding ProjectNational Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11775272] ; Central-level scientific institutions[Y79XG13361]
Funding OrganizationNational Natural Science Foundation of China ; Central-level scientific institutions
WOS Research AreaToxicology
WOS SubjectToxicology
WOS IDWOS:000671151100018
Citation statistics
Document Type期刊论文
Corresponding AuthorZhang, Jing-Song; Huang, Qing
Affiliation1.Chinese Acad Sci, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei 230031, Peoples R China
2.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China
3.Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Beijing, Peoples R China
4.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Inst Edible Fungi, Shanghai 201403, Peoples R China
Recommended Citation
GB/T 7714
Shao, Chang-Sheng,Feng, Na,Zhou, Shuai,et al. Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis[J]. TOXICOLOGY RESEARCH,2021,10.
APA Shao, Chang-Sheng.,Feng, Na.,Zhou, Shuai.,Zheng, Xin-Xin.,Wang, Peng.,...&Huang, Qing.(2021).Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis.TOXICOLOGY RESEARCH,10.
MLA Shao, Chang-Sheng,et al."Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis".TOXICOLOGY RESEARCH 10(2021).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shao, Chang-Sheng]'s Articles
[Feng, Na]'s Articles
[Zhou, Shuai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shao, Chang-Sheng]'s Articles
[Feng, Na]'s Articles
[Zhou, Shuai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shao, Chang-Sheng]'s Articles
[Feng, Na]'s Articles
[Zhou, Shuai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.