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Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis
Shao, Chang-Sheng1,2; Feng, Na3,4; Zhou, Shuai3; Zheng, Xin-Xin1,2; Wang, Peng1; Zhang, Jing-Song3,4; Huang, Qing1,2
2021-05-01
Source PublicationTOXICOLOGY RESEARCH
ISSN2045-452X
Corresponding AuthorZhang, Jing-Song(syja16@saas.sh.cn) ; Huang, Qing(huangq@ipp.ac.cn)
AbstractThe use of natural substances derived from traditional Chinese medicine and natural plants as safe radiosensitizing adjuvants is a new trend for cancer radiotherapy. Ganoderma lucidum has been used as a traditional Chinese medicine with a history of more than 2000 years. Ganoderic acid T (GAT) is a typical triterpene of G. lucidum, which has strong cytotoxicity to cancer cells, but whether it has radiation sensitization effect has not been explored. In this work, we treated the HeLa cells with different concentrations of GAT before exposure to gamma-ray radiation and investigated its influence on the radiosensitivity. The cell viability, apoptosis rate, necoptosis rate, intracellular ATP level, cell cycle, the amount of H2AX and 53BP1, reactive oxygen species, and mitochondrial membrane potential were examined. Apoptotic, necroptotic, and autophagic biomarker proteins, including caspase 8, cytochrome c, caspase 3, RIPK, MLKL, P62, and LC3, were analyzed. As a result, we confirmed that with treatment of GAT, the gamma-ray radiation induced both apoptosis and necroptosis in HeLa cells, and with increase of GAT, the percentage ratio of necroptosis was increased. The involved pathways and mechanisms were also explored and discussed.
Keywordganoderic acid T (GAT) triterpene apoptosis necroptosis radiosensitivity ionizing radiation
DOI10.1093/toxres/tfab030
WOS KeywordCANCER-CELLS ; DEATH FATE ; INHIBITION ; LUCIDUM ; SWITCH
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11775272] ; Central-level scientific institutions[Y79XG13361]
Funding OrganizationNational Natural Science Foundation of China ; Central-level scientific institutions
WOS Research AreaToxicology
WOS SubjectToxicology
WOS IDWOS:000671151100018
PublisherOXFORD UNIV PRESS
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Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/123506
Collection中国科学院合肥物质科学研究院
Corresponding AuthorZhang, Jing-Song; Huang, Qing
Affiliation1.Chinese Acad Sci, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei 230031, Peoples R China
2.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China
3.Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Beijing, Peoples R China
4.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Inst Edible Fungi, Shanghai 201403, Peoples R China
Recommended Citation
GB/T 7714
Shao, Chang-Sheng,Feng, Na,Zhou, Shuai,et al. Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis[J]. TOXICOLOGY RESEARCH,2021,10.
APA Shao, Chang-Sheng.,Feng, Na.,Zhou, Shuai.,Zheng, Xin-Xin.,Wang, Peng.,...&Huang, Qing.(2021).Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis.TOXICOLOGY RESEARCH,10.
MLA Shao, Chang-Sheng,et al."Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis".TOXICOLOGY RESEARCH 10(2021).
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