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| Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis | |
Shao, Chang-Sheng1,2; Feng, Na3,4; Zhou, Shuai3; Zheng, Xin-Xin1,2; Wang, Peng1; Zhang, Jing-Song3,4; Huang, Qing1,2
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| 2021-05-01 | |
| Source Publication | TOXICOLOGY RESEARCH
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| ISSN | 2045-452X |
| Corresponding Author | Zhang, Jing-Song(syja16@saas.sh.cn) ; Huang, Qing(huangq@ipp.ac.cn) |
| Abstract | The use of natural substances derived from traditional Chinese medicine and natural plants as safe radiosensitizing adjuvants is a new trend for cancer radiotherapy. Ganoderma lucidum has been used as a traditional Chinese medicine with a history of more than 2000 years. Ganoderic acid T (GAT) is a typical triterpene of G. lucidum, which has strong cytotoxicity to cancer cells, but whether it has radiation sensitization effect has not been explored. In this work, we treated the HeLa cells with different concentrations of GAT before exposure to gamma-ray radiation and investigated its influence on the radiosensitivity. The cell viability, apoptosis rate, necoptosis rate, intracellular ATP level, cell cycle, the amount of H2AX and 53BP1, reactive oxygen species, and mitochondrial membrane potential were examined. Apoptotic, necroptotic, and autophagic biomarker proteins, including caspase 8, cytochrome c, caspase 3, RIPK, MLKL, P62, and LC3, were analyzed. As a result, we confirmed that with treatment of GAT, the gamma-ray radiation induced both apoptosis and necroptosis in HeLa cells, and with increase of GAT, the percentage ratio of necroptosis was increased. The involved pathways and mechanisms were also explored and discussed. |
| Keyword | ganoderic acid T (GAT) triterpene apoptosis necroptosis radiosensitivity ionizing radiation |
| DOI | 10.1093/toxres/tfab030 |
| WOS Keyword | CANCER-CELLS ; DEATH FATE ; INHIBITION ; LUCIDUM ; SWITCH |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11775272] ; Central-level scientific institutions[Y79XG13361] |
| Funding Organization | National Natural Science Foundation of China ; Central-level scientific institutions |
| WOS Research Area | Toxicology |
| WOS Subject | Toxicology |
| WOS ID | WOS:000671151100018 |
| Publisher | OXFORD UNIV PRESS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.hfcas.ac.cn:8080/handle/334002/123506 |
| Collection | 中国科学院合肥物质科学研究院 |
| Corresponding Author | Zhang, Jing-Song; Huang, Qing |
| Affiliation | 1.Chinese Acad Sci, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China 3.Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Beijing, Peoples R China 4.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Inst Edible Fungi, Shanghai 201403, Peoples R China |
| Recommended Citation GB/T 7714 | Shao, Chang-Sheng,Feng, Na,Zhou, Shuai,et al. Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis[J]. TOXICOLOGY RESEARCH,2021,10. |
| APA | Shao, Chang-Sheng.,Feng, Na.,Zhou, Shuai.,Zheng, Xin-Xin.,Wang, Peng.,...&Huang, Qing.(2021).Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis.TOXICOLOGY RESEARCH,10. |
| MLA | Shao, Chang-Sheng,et al."Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis".TOXICOLOGY RESEARCH 10(2021). |
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