Knowledge Management System of Hefei Institute of Physical Science,CAS
Simultaneous detection of tumor-related mRNA and miRNA in cancer cells with magnetic SERS nanotags | |
Li, Man1; Li, Jingya2,3; Zhang, Xiang1; Yao, Mingming1; Li, Pan4; Xu, Weiping1,5 | |
2021-09-01 | |
发表期刊 | TALANTA |
ISSN | 0039-9140 |
通讯作者 | Li, Pan(lipan2011@iim.ac.cn) ; Xu, Weiping(weipingx@ustc.edu.cn) |
摘要 | The design of simultaneous detection method has broad prospects for cancer diagnosis and prognosis. Herein, we reported a low cost and sensitive SERS sensing platform for simultaneous p21 mRNA and miRNA-21 detection based on duplex-specific nuclease signal amplification (DSNSA) plus multifunctional Fe3O4@SiO2 magnetic nanoparticles (Fe3O4@SiO2 MNPs). Here, Fe3O4@SiO2 MNPs were used as a separation substrate, and Au@AgNPs served as stable and ultrasensitive SERS nanotags. Firstly, Au@AgNPs and Fe3O4@SiO2 MNPs were attached to both ends of capture probe (CP) by covalent bonds. Under the assistance of the target p21 mRNA and miRNA-21, DNA (CP) of the DNA-RNA heteroduplexes could be specifically degraded by DSN and the SERS nanotags that were released from the surface of Fe3O4@SiO2 MNPs. Meanwhile, the target p21 mRNA and miRNA-21 were released and then involved in the next round of signal reactions. The proposed strategy allowed quantitative detection of p21 mRNA and miRNA-21 and the limit of detection (LOD) was 0.12 fM and 0.17 fM, respectively. This method gives a great potential for multiplex detection of biological molecules. |
关键词 | p21 mRNA microRNA-21 Duplex-specific nuclease signal amplification (DSNSA) Simultaneous detection The SERS-Based platform |
DOI | 10.1016/j.talanta.2021.122432 |
关键词[WOS] | DUPLEX-SPECIFIC NUCLEASE ; SIGNAL AMPLIFICATION STRATEGY ; MICRORNAS ; CORE ; NANOPARTICLES ; EXPRESSION ; MIR-21 ; SHELL |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[51672004] ; Natural Science Foundation of Anhui Province (China)[1608085MH176] ; Natural Science Foundation of Anhui Province (China)[1908085QB65] ; Key Research and Development Program of Anhui Province (China)[1804h08020252] |
项目资助者 | National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province (China) ; Key Research and Development Program of Anhui Province (China) |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000658317000039 |
出版者 | ELSEVIER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/123745 |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Li, Pan; Xu, Weiping |
作者单位 | 1.Univ Sci & Technol China, Affiliated Hosp USTC 1, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Dept Biol Phys, Hefei 230026, Peoples R China 4.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Hefei 230021, Peoples R China 5.Anhui Prov Key Lab Tumor Immunotherapy & Nutr The, Hefei 230001, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Man,Li, Jingya,Zhang, Xiang,et al. Simultaneous detection of tumor-related mRNA and miRNA in cancer cells with magnetic SERS nanotags[J]. TALANTA,2021,232. |
APA | Li, Man,Li, Jingya,Zhang, Xiang,Yao, Mingming,Li, Pan,&Xu, Weiping.(2021).Simultaneous detection of tumor-related mRNA and miRNA in cancer cells with magnetic SERS nanotags.TALANTA,232. |
MLA | Li, Man,et al."Simultaneous detection of tumor-related mRNA and miRNA in cancer cells with magnetic SERS nanotags".TALANTA 232(2021). |
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