Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro

doi:10.1002/tox.23381

HFCAS OpenIR

	Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro
	Wang, Caixia 1; Gu, Zhangyu 2,3,4; Gu, Xueyan 1; Tan, Xinyue 1; Wang, Shuang 1; Zhang, Rui1 ; Li, Ruifen 1; Sun, Mingkun 1; Gui, Chunyan 1; Li, Sheng5 ; Ye, Yixing3,4 ; Ma, Jianhua 1; Su, Li 1,6; Liang, Changhao3,4
	2021-10-06
发表期刊	ENVIRONMENTAL TOXICOLOGY
ISSN	1520-4081
通讯作者	Su, Li(sul@lzu.edu.cn) ; Liang, Changhao(chliang@issp.ac.cn)
摘要	The aim of this study was to investigate the protective effects of Nano-Se against nickel (Ni)-induced hepatotoxicity and the potential mechanism. Hence, we constructed in vivo and in vitro models of Ni-induced hepatotoxicity. Sprague-Dawley (SD) rats were exposed to nickel sulfate (NiSO4, 5.0 mg/kg, i.p.) with or without Nano-Se (0.5, 1, and 2 mg/kg, oral gavage) co-administration for 14 days, and HepG2 cells were exposed to NiSO4 (1500 mu M) with or without Nano-Se (20 mu M) for 24 h. Nano-Se obviously prevented Ni-induced hepatotoxicity indicated by ameliorating pathological change and decreasing Ni accumulation in rat livers. Ni induced a significant increase in hepatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GSH-Px), and malondialdehyde (MDA) level, decreased the glutathione (GSH) content while compared to those in the control group. Nano-Se administration improved the hepatic antioxidant capacity through increase hepatic GSH contents and GSH-Px activity, decrease the activities of SOD, CAT, and MDA level. Nano-Se improved the cell viability, decreased active oxygen (ROS) generation and ameliorated morphological changes of nuclear structures in Ni-treated HepG2 cells. In addition, Nano-Se inhibited the Ni-induced increases of cytochrome c, caspase-9, cleaved caspase-3, increased PI3K and AKT phosphorylation both in vivo and in vitro. Besides, the PI3K inhibitor Y294002 could inhibit the protective effects of Nano-Se on apoptosis. Thus, Nano-Se significantly activates PI3K/AKT signaling to ameliorate apoptosis in Ni-induced hepatotoxicity.
关键词	hepatotoxicity mitochondrial apoptosis Nano-Se nickel PI3K-Akt pathway
DOI	10.1002/tox.23381
关键词[WOS]	PROTECTS MOUSE-LIVER ; RETICULUM STRESS PATHWAYS ; INDUCED OXIDATIVE STRESS ; CELL-CYCLE ARREST ; ENDOPLASMIC-RETICULUM ; NANOPARTICLES ; TOXICITY ; CYTOTOXICITY ; ANTIOXIDANT ; DYSFUNCTION
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81402703] ; Natural Science Foundation of Gansu Province[20JR5RA24B] ; Fundamental Research Funds for the Central Universities[lzu-jbky-2019-7] ; National Innovation and Entrepreneurship Training Scheme of Lanzhou University[202010730240]
项目资助者	National Natural Science Foundation of China ; Natural Science Foundation of Gansu Province ; Fundamental Research Funds for the Central Universities ; National Innovation and Entrepreneurship Training Scheme of Lanzhou University
WOS研究方向	Environmental Sciences & Ecology ; Toxicology ; Water Resources
WOS类目	Environmental Sciences ; Toxicology ; Water Resources
WOS记录号	WOS:000703955400001
出版者	WILEY
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/124904
专题	中国科学院合肥物质科学研究院
通讯作者	Su, Li; Liang, Changhao
作者单位	1.Lanzhou Univ, Sch Publ Hlth, Lanzhou 730000, Peoples R China 2.Zhengzhou Univ, Henan Inst Adv Technol, Zhengzhou, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Solid State Phys, Key Lab Mat Phys, Hefei 230031, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Solid State Phys, Anhui Key Lab Nanomat & Nanotechnol, Hefei 230031, Peoples R China 5.First Peoples Hosp Lanzhou City, Lanzhou, Peoples R China 6.Key Lab Biol Monitoring & Restorat Environm Pollu, Lanzhou, Peoples R China
通讯作者单位	中科院固体物理研究所
推荐引用方式 GB/T 7714	Wang, Caixia,Gu, Zhangyu,Gu, Xueyan,et al. Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro[J]. ENVIRONMENTAL TOXICOLOGY,2021.
APA	Wang, Caixia.,Gu, Zhangyu.,Gu, Xueyan.,Tan, Xinyue.,Wang, Shuang.,...&Liang, Changhao.(2021).Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro.ENVIRONMENTAL TOXICOLOGY.
MLA	Wang, Caixia,et al."Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro".ENVIRONMENTAL TOXICOLOGY (2021).