Knowledge Management System of Hefei Institute of Physical Science，CAS
| Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro | |
Wang, Caixia1; Gu, Zhangyu2,3,4; Gu, Xueyan1; Tan, Xinyue1; Wang, Shuang1; Zhang, Rui1 ; Li, Ruifen1; Sun, Mingkun1; Gui, Chunyan1; Li, Sheng5; Ye, Yixing3,4; Ma, Jianhua1; Su, Li1,6; Liang, Changhao3,4
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| 2021-10-06 | |
| Source Publication | ENVIRONMENTAL TOXICOLOGY
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| ISSN | 1520-4081 |
| Corresponding Author | Su, Li(sul@lzu.edu.cn) ; Liang, Changhao(chliang@issp.ac.cn) |
| Abstract | The aim of this study was to investigate the protective effects of Nano-Se against nickel (Ni)-induced hepatotoxicity and the potential mechanism. Hence, we constructed in vivo and in vitro models of Ni-induced hepatotoxicity. Sprague-Dawley (SD) rats were exposed to nickel sulfate (NiSO4, 5.0 mg/kg, i.p.) with or without Nano-Se (0.5, 1, and 2 mg/kg, oral gavage) co-administration for 14 days, and HepG2 cells were exposed to NiSO4 (1500 mu M) with or without Nano-Se (20 mu M) for 24 h. Nano-Se obviously prevented Ni-induced hepatotoxicity indicated by ameliorating pathological change and decreasing Ni accumulation in rat livers. Ni induced a significant increase in hepatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GSH-Px), and malondialdehyde (MDA) level, decreased the glutathione (GSH) content while compared to those in the control group. Nano-Se administration improved the hepatic antioxidant capacity through increase hepatic GSH contents and GSH-Px activity, decrease the activities of SOD, CAT, and MDA level. Nano-Se improved the cell viability, decreased active oxygen (ROS) generation and ameliorated morphological changes of nuclear structures in Ni-treated HepG2 cells. In addition, Nano-Se inhibited the Ni-induced increases of cytochrome c, caspase-9, cleaved caspase-3, increased PI3K and AKT phosphorylation both in vivo and in vitro. Besides, the PI3K inhibitor Y294002 could inhibit the protective effects of Nano-Se on apoptosis. Thus, Nano-Se significantly activates PI3K/AKT signaling to ameliorate apoptosis in Ni-induced hepatotoxicity. |
| Keyword | hepatotoxicity mitochondrial apoptosis Nano-Se nickel PI3K-Akt pathway |
| DOI | 10.1002/tox.23381 |
| WOS Keyword | PROTECTS MOUSE-LIVER ; RETICULUM STRESS PATHWAYS ; INDUCED OXIDATIVE STRESS ; CELL-CYCLE ARREST ; ENDOPLASMIC-RETICULUM ; NANOPARTICLES ; TOXICITY ; CYTOTOXICITY ; ANTIOXIDANT ; DYSFUNCTION |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[81402703] ; Natural Science Foundation of Gansu Province[20JR5RA24B] ; Fundamental Research Funds for the Central Universities[lzu-jbky-2019-7] ; National Innovation and Entrepreneurship Training Scheme of Lanzhou University[202010730240] |
| Funding Organization | National Natural Science Foundation of China ; Natural Science Foundation of Gansu Province ; Fundamental Research Funds for the Central Universities ; National Innovation and Entrepreneurship Training Scheme of Lanzhou University |
| WOS Research Area | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| WOS Subject | Environmental Sciences ; Toxicology ; Water Resources |
| WOS ID | WOS:000703955400001 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.hfcas.ac.cn:8080/handle/334002/124904 |
| Collection | 中国科学院合肥物质科学研究院 |
| Corresponding Author | Su, Li; Liang, Changhao |
| Affiliation | 1.Lanzhou Univ, Sch Publ Hlth, Lanzhou 730000, Peoples R China 2.Zhengzhou Univ, Henan Inst Adv Technol, Zhengzhou, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Solid State Phys, Key Lab Mat Phys, Hefei 230031, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Solid State Phys, Anhui Key Lab Nanomat & Nanotechnol, Hefei 230031, Peoples R China 5.First Peoples Hosp Lanzhou City, Lanzhou, Peoples R China 6.Key Lab Biol Monitoring & Restorat Environm Pollu, Lanzhou, Peoples R China |
| Corresponding Author Affilication | Chinese Acad Sci, Inst Solid State Phys， Hefei 230031, Anhui, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Caixia,Gu, Zhangyu,Gu, Xueyan,et al. Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro[J]. ENVIRONMENTAL TOXICOLOGY,2021. |
| APA | Wang, Caixia.,Gu, Zhangyu.,Gu, Xueyan.,Tan, Xinyue.,Wang, Shuang.,...&Liang, Changhao.(2021).Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro.ENVIRONMENTAL TOXICOLOGY. |
| MLA | Wang, Caixia,et al."Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro".ENVIRONMENTAL TOXICOLOGY (2021). |
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