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| Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2 | |
Yang, Qiang1,2; Zheng, Zhiming1 ; Zhao, Genhai1; Wang, Li1,2; Wang, Han1; Ni, Wenfeng1,2; Sun, Xiaowen1,2; Zhang, Mengxue1,2; Tang, Hengfang1,2; Wang, Peng1
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| 2022 | |
| Source Publication | PROTEIN EXPRESSION AND PURIFICATION
 |
| ISSN | 1046-5928 |
| Corresponding Author | Zheng, Zhiming(zhengzhiming2014@163.com) ; Wang, Peng(pengwang@ipp.ac.cn) |
| Abstract | To date, there is no functional characterization of EmGGPPS (from Elizabethkingia meningoseptica sp.F2) as enzymes catalyzing GGPP. In this research, maltose-binding protein (MBP), disulfide bond A (DbsA), disulfide bond C (DbsC), and two other small protein tags, GB1 (Protein G B1 domain) and ZZ (Protein A IgG ZZ repeat domain), were used as fusion partners to construct an EmGGPPS fusion expression system. The results indicated that the expression of MBP-EmGGPPS was higher than that of the other four fusion proteins in E. coli BL21 (DE3). Additionally, using EmGGPPS as a catalyst for the production of GGPP was verified using a color complementation assay in Escherichia coli. In parallel with it, the enzyme activity experiment in vitro showed that the EmGGPPS protein could produce GGPP, GPP and FPP. Finally, we successfully demonstrated MK-4 production in engineered E. coli by overexpression of EmGGPPS. |
| Keyword | Elizabethkingia meningoseptica Geranylgeranyl diphosphate synthase Soluble expression MK-4 |
| DOI | 10.1016/j.pep.2021.105986 |
| WOS Keyword | ESCHERICHIA-COLI ; ISOPRENOID BIOSYNTHESIS ; PROTEIN EXPRESSION ; BINDING DOMAIN ; VITAMIN-K ; PURIFICATION ; SYNTHASE ; PATHWAY ; YEAST |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS Subject | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS ID | WOS:000709874400002 |
| Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.hfcas.ac.cn:8080/handle/334002/125763 |
| Collection | 中国科学院合肥物质科学研究院 |
| Corresponding Author | Zheng, Zhiming; Wang, Peng |
| Affiliation | 1.Chinese Acad Sci, Inst Intelligent Machines, Hefei Inst Phys Sci, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, Qiang,Zheng, Zhiming,Zhao, Genhai,et al. Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2[J]. PROTEIN EXPRESSION AND PURIFICATION,2022,189. |
| APA | Yang, Qiang.,Zheng, Zhiming.,Zhao, Genhai.,Wang, Li.,Wang, Han.,...&Wang, Peng.(2022).Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2.PROTEIN EXPRESSION AND PURIFICATION,189. |
| MLA | Yang, Qiang,et al."Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2".PROTEIN EXPRESSION AND PURIFICATION 189(2022). |
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