Hemin enhances radiosensitivity of lung cancer cells through ferroptosis

doi:10.1016/j.yexcr.2021.112946

HFCAS OpenIR

	Hemin enhances radiosensitivity of lung cancer cells through ferroptosis
	Almahi, Waleed Abdelbag 1,2,3; Yu, K. N.4,5; Mohammed, Fathelrahman 6; Kong, Peizhong 1; Han, Wei1,7
	2022
发表期刊	EXPERIMENTAL CELL RESEARCH
ISSN	0014-4827
通讯作者	Han, Wei(hanw@hfcas.ac.cn)
摘要	The principle underlying radiotherapy is to kill cancer cells while minimizing the harmful effects on non-cancer cells, which has still remained as a major challenge. In relation, ferroptosis has recently been proposed as a novel mechanism of radiation-induced cell death. In this study, we investigated and demonstrated the role of Hemin as an iron overloading agent in the generation of reactive oxygen species (ROS) induced by ionizing radiation in lung cancer and non-cancer cells. It was found that the presence of Hemin in irradiated lung cancer cells enhanced the productivity of initial ROS, resulting in lipid peroxidation and subsequent ferroptosis. We observed that application of Hemin as a co-treatment increased the activity of GPx4 degradation in both cancer and normal lung cells. Furthermore, Hemin protected normal lung cells against radiation-induced cell death, in that it suppressed ROS after radiation, and boosted the production of bilirubin which was a lipophilic ROS antioxidant. In addition, we demonstrated significant FTH1 expression in normal lung cells when compared to lung cancer cells, which prevented iron from playing a role in increasing IR-induced cell death. Our findings demonstrated that Hemin had a dual function in enhancing the radiosensitivity of ferroptosis in lung cancer cells while promoting cell survival in normal lung cells.
关键词	Ferroptosis Hemin Radiosensitivity ROS GPx4 FTH1 Bilirubin
DOI	10.1016/j.yexcr.2021.112946
关键词[WOS]	OXIDATIVE STRESS-RESPONSE ; FERRITIN ; GLUTATHIONE ; METABOLISM ; BILIRUBIN ; PATHWAY ; GPX4
收录类别	SCI
语种	英语
资助项目	Chinese National Natural Science Foundation of China[81974484] ; CASHIPS Directors Fund[YZJJ2018QN19] ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Lab in Marine Pollution (SKLMP), City University of Hong Kong[IRF/0024]
项目资助者	Chinese National Natural Science Foundation of China ; CASHIPS Directors Fund ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Lab in Marine Pollution (SKLMP), City University of Hong Kong
WOS研究方向	Oncology ; Cell Biology
WOS类目	Oncology ; Cell Biology
WOS记录号	WOS:000779968900003
出版者	ELSEVIER INC
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/128598
专题	中国科学院合肥物质科学研究院
通讯作者	Han, Wei
作者单位	1.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Grad Sch, Sci Isl Branch, Hefei 230026, Peoples R China 3.Sudan Atom Energy Commiss, Nucl Applicat Biol Sci Inst, Radiobiol & Canc Res Dept, POB 3001, Khartoum 11111, North Sudan 4.City Univ Hong Kong, Dept Phys, Kowloon Tong, Tat Chee Ave, Hong Kong 999077, Peoples R China 5.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon Tong, Tat Chee Ave, Hong Kong 999077, Peoples R China 6.Univ Sci & Technol China, Dept Polymer Sci & Engn, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China 7.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Almahi, Waleed Abdelbag,Yu, K. N.,Mohammed, Fathelrahman,et al. Hemin enhances radiosensitivity of lung cancer cells through ferroptosis[J]. EXPERIMENTAL CELL RESEARCH,2022,410.
APA	Almahi, Waleed Abdelbag,Yu, K. N.,Mohammed, Fathelrahman,Kong, Peizhong,&Han, Wei.(2022).Hemin enhances radiosensitivity of lung cancer cells through ferroptosis.EXPERIMENTAL CELL RESEARCH,410.
MLA	Almahi, Waleed Abdelbag,et al."Hemin enhances radiosensitivity of lung cancer cells through ferroptosis".EXPERIMENTAL CELL RESEARCH 410(2022).