Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation

doi:10.1007/s00253-022-12174-9

HFCAS OpenIR

	Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation
	Jiang, Chunxu 1,2; Wang, Han 1; Liu, Menghan 3; Wang, Li 1; Yang, Ruwen 3; Wang, Peng1 ; Lu, Zongmei 3; Zhou, Yong 3; Zheng, Zhiming1 ; Zhao, Genhai1
	2022-09-23
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN	0175-7598
通讯作者	Wang, Han(wanghan@ipp.ac.cn) ; Zhou, Yong(Zy197579@163.com) ; Zheng, Zhiming(zhengzhiming2014@163.com) ; Zhao, Genhai(zhgh327@ipp.ac.cn)
摘要	The biosynthesis of citric acid (CA) using Aspergillus niger as a carrier is influenced by mycelium morphology, which is determined by the expression level of morphology-related genes. As a key component of the fungal cell wall, chitin content has an important effect on morphogenesis, and to investigate the effects of this on fermentation performance, we used RNA interference to knockdown chitin synthase C (CHSC) and chitin synthase activator (CHS3) to obtain the single-gene mutant strains A. niger chs3 and chsC and the double mutant A. niger chs3C. We found that the CA fermentation performance of the two single mutants was significantly better than that of the double mutant. The mutant A. niger chs3-4 exhibited CA production potential compared to that of the parent strain in scale-up fermentation; we determined certain characteristics of CA high-yielding strain fermentation pellets. In addition, when chsC alone was silenced, there was very little change in chs3 mRNA levels, whereas those of chsC were significantly reduced when only chs3 was silenced. As this may be because of a synergistic effect between chsC and chs3, and we speculated that the latent activation target of CHS3 is CHSC, our results confirmed this hypothesis. This study is the first application of a separation and combination silence strategy of chitin synthase and chitin synthase activator in the morphology of A. niger CA fermentation. Furthermore, it provides new insights into the method for the morphological study of A. niger fermentation and the interaction of homologous genes.
关键词	Chitin synthases activator Citric acid Morphology Aspergillus niger RNAi Synergistic effect
DOI	10.1007/s00253-022-12174-9
关键词[WOS]	SACCHAROMYCES-CEREVISIAE ; CELL-WALL ; SEPTUM FORMATION ; YEAST ; GENES ; EXPRESSION ; DOMAIN ; PROTEINS ; GENETICS ; DIVISION
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2019YFA0904304] ; National Natural Science Foundation of China[32070088] ; National Natural Science Foundation of Anhui China[1908085MB43] ; Major Projects of Science and Technology in Anhui Province[202103a06020003]
项目资助者	National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Anhui China ; Major Projects of Science and Technology in Anhui Province
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000857274300002
出版者	SPRINGER
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/128983
专题	中国科学院合肥物质科学研究院
通讯作者	Wang, Han; Zhou, Yong; Zheng, Zhiming; Zhao, Genhai
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Comprehens Lab Bldg,350 Shushanhu Rd,POB 1138, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei, Anhui, Peoples R China 3.COFCO Biotechnol Co Ltd, 1 Zhongliang Ave, Bengbu 233010, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Chunxu,Wang, Han,Liu, Menghan,et al. Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2022.
APA	Jiang, Chunxu.,Wang, Han.,Liu, Menghan.,Wang, Li.,Yang, Ruwen.,...&Zhao, Genhai.(2022).Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY.
MLA	Jiang, Chunxu,et al."Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2022).