Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield

doi:10.1155/2022/8407829

HFCAS OpenIR

	Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield
	Li, Chu 1,2; Wang, Han 1; Ma, Guoliang 1; Wang, Li 1; Zheng, Zhiming1 ; Zhao, Genhai1 ; Wang, Peng 1
	2022-09-16
发表期刊	JOURNAL OF FOOD QUALITY
ISSN	0146-9428
通讯作者	Wang, Han(wanghan@ipp.ac.cn) ; Zheng, Zhiming(zhengzhiming2014@163.com)
摘要	Bacillus subtilis is one of the few strains that can secrete synthetic menaquinone-7 (MK-7) to the outside of the cell, and its purpose and mechanism have not been clearly studied. As an amphiphilic protein naturally synthesized by Bacillus subtilis, the BslA protein may be involved in the inversion of extracellular vitamin K2 solubility. The protein structure in UniProt was used to search for the possible binding sites of MK-7, and the analysis of the higher ranking results of the genetic algorithm showed that the ASP166 residue was likely to be the binding site. They could form a stable hydrogen bond connection through ASP166, and approximately 7 proteins formed the conformation of a fixed naphthoquinone ring. We isolated and obtained the BslA protein by Ni-NTA affinity chromatography. Then, MK-7 was modified by BslA in vitro. A series of experiments, such as SEM, XPS, and WCA, showed that MK-7 and BslA proteins can realize self-assembly and transform from fat-soluble to water-soluble complexes. When the bslA protein in Bacillus subtilis natto was overexpressed, its MK-7 synthesis ability was further improved, especially the extracellular MK-7 content, which increased by 16%. This finding suggests that the BslA protein in Bacillus subtilis is likely to be involved in the extracellular secretion of MK-7 as a receptor.
DOI	10.1155/2022/8407829
关键词[WOS]	MENAQUINONE-7 ; CELLS
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2019YFA0904304] ; National Natural Science Foundation of Anhui China[1908085MB43] ; Major Projects of Science and Technology in Anhui Province[202103a06020003] ; National Natural Science Foundation of China[32070088]
项目资助者	National Key Research and Development Program of China ; National Natural Science Foundation of Anhui China ; Major Projects of Science and Technology in Anhui Province ; National Natural Science Foundation of China
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:000861569300003
出版者	WILEY-HINDAWI
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/129108
专题	中国科学院合肥物质科学研究院
通讯作者	Wang, Han; Zheng, Zhiming
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei, Peoples R China 2.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Li, Chu,Wang, Han,Ma, Guoliang,et al. Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield[J]. JOURNAL OF FOOD QUALITY,2022,2022.
APA	Li, Chu.,Wang, Han.,Ma, Guoliang.,Wang, Li.,Zheng, Zhiming.,...&Wang, Peng.(2022).Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield.JOURNAL OF FOOD QUALITY,2022.
MLA	Li, Chu,et al."Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield".JOURNAL OF FOOD QUALITY 2022(2022).