A dynamic fluorescence probed glycolysis suppression process in HeLa cells treated with trichostatin A

doi:10.1039/d2an01114j

HFCAS OpenIR

	A dynamic fluorescence probed glycolysis suppression process in HeLa cells treated with trichostatin A
	Zhang, Fengqiu 1,2; Shao, Changsheng 2,3; Wu, Yahui 2,3; Zhao, Wei 1; Jing, Xumiao 1; Fang, Cao 2,3; Huang, Qing2,3
	2022-09-21
发表期刊	ANALYST
ISSN	0003-2654
通讯作者	Huang, Qing(huangq@ipp.ac.cn)
摘要	Acetylation can be regulated by histone deacetylases (HDACs) and histone acetyltransferases (HATs), and the imbalance between HDACs and HATs can lead to cancers. Trichostatin A (TSA), as one of the typical HDAC inhibitors (HDACis), may regulate the acetylation level and thus prevent the proliferation of cancer cells, in which the metabolic change of glycolysis is involved. However, the dynamic process of glycolysis has not yet been explored, and the mechanism is still elusive. In this work, we constructed GFP-actin-HeLa cells to observe the dynamic change of glycolysis in situ and found that the GFP fluorescence enhanced significantly after TSA treatment, which was consistent with the change of pH in the cells (pH(i)) depending on the change of lactate originated from glycolysis. Furthermore, we confirmed that the glycolysis was inhibited after TSA treatment, and this process was associated with HIF-1 alpha and c-Myc regulation. As such, this work not only demonstrates the usefulness of the GFP fluorescent probe for monitoring the metabolic process of glycolysis in situ, but also sheds new light on the mechanism of glycolysis suppression in the cancer cells treated with HDACi.
DOI	10.1039/d2an01114j
关键词[WOS]	HISTONE DEACETYLASE INHIBITORS ; AEROBIC GLYCOLYSIS ; GENE ; ACETYLATION ; METABOLISM ; LACTATE ; PROTEIN ; PH ; HIF-1-ALPHA ; EXPRESSION
收录类别	SCI
语种	英语
资助项目	National Nature Science Foundation of China[11635013] ; College Key Research Project in Henan Province[22A180030] ; Research Integration Program of Hefei Institutes of Physical Science, Chinese Academy of Sciences
项目资助者	National Nature Science Foundation of China ; College Key Research Project in Henan Province ; Research Integration Program of Hefei Institutes of Physical Science, Chinese Academy of Sciences
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000870356500001
出版者	ROYAL SOC CHEMISTRY
引用统计
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/129445
专题	中国科学院合肥物质科学研究院
通讯作者	Huang, Qing
作者单位	1.Zhengzhou Univ, Sch Phys & Microelect, Henan Key Lab Ion Beam Bioengn, Zhengzhou, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei, Peoples R China 3.Univ Sci & Technol China, Grad Sch, Sci Isl Branch, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Fengqiu,Shao, Changsheng,Wu, Yahui,et al. A dynamic fluorescence probed glycolysis suppression process in HeLa cells treated with trichostatin A[J]. ANALYST,2022.
APA	Zhang, Fengqiu.,Shao, Changsheng.,Wu, Yahui.,Zhao, Wei.,Jing, Xumiao.,...&Huang, Qing.(2022).A dynamic fluorescence probed glycolysis suppression process in HeLa cells treated with trichostatin A.ANALYST.
MLA	Zhang, Fengqiu,et al."A dynamic fluorescence probed glycolysis suppression process in HeLa cells treated with trichostatin A".ANALYST (2022).