Simplified Cell Magnetic Isolation Assisted SC2 Chip to Realize "Sample in and Chemotaxis Out": Validated by Healthy and T2DM Patients' Neutrophils

doi:10.3390/mi13111820

HFCAS OpenIR

	Simplified Cell Magnetic Isolation Assisted SC2 Chip to Realize "Sample in and Chemotaxis Out": Validated by Healthy and T2DM Patients' Neutrophils
	Yang, Xiao 1,2; Gao, Chaoru 1,2; Liu, Yong 1; Zhu, Ling1 ; Yang, Ke1
	2022-11-01
发表期刊	MICROMACHINES
通讯作者	Zhu, Ling(zhul@aiofm.ac.cn) ; Yang, Ke(keyang@aiofm.ac.cn)
摘要	Neutrophil migration in tissues critically regulates the human immune response and can either play a protective role in host defense or cause health problems. Microfluidic chips are increasingly applied to study neutrophil migration, attributing to their advantages of low reagent consumption, stable chemical gradients, visualized cell chemotaxis monitoring, and quantification. Most chemotaxis chips suffered from low throughput and fussy cell separation operations. We here reported a novel and simple "sample in and chemotaxis out" method for rapid neutrophils isolation from a small amount of whole blood based on a simplified magnetic method, followed by a chemotaxis assay on a microfluidic chip (SC2 chip) consisting of six cell migration units and six-cell arrangement areas. The advantages of the "sample in and chemotaxis out" method included: less reagent consumption (10 mu L of blood + 1 mu L of magnetic beads + 1 mu L of lysis buffer); less time (5 min of cell isolation + 15 min of chemotaxis testing); no ultracentrifugation; more convenient; higher efficiency; high throughput. We have successfully validated the approach by measuring neutrophil chemotaxis to frequently-used chemoattractant (i.e., fMLP). The effects of D-glucose and mannitol on neutrophil chemotaxis were also analyzed. In addition, we demonstrated the effectiveness of this approach for testing clinical samples from diabetes mellitus type 2 (T2DM) patients. We found neutrophils' migration speed was higher in the "well-control" T2DM than in the "poor-control" group. Pearson coefficient analysis further showed that the migration speed of T2DM was negatively correlated with physiological indicators, such as HbA1c (-0.44), triglyceride (-0.36), C-reactive protein (-0.28), and total cholesterol (-0.28). We are very confident that the developed "sample in and chemotaxis out" method was hoped to be an attractive model for analyzing the chemotaxis of healthy and disease-associated neutrophils.
关键词	neutrophil microfluidic chip isolation chemotaxis test diabetes mellitus type 2 (T2DM)
DOI	10.3390/mi13111820
关键词[WOS]	MIGRATION ASSAY ; DROP ; BLOOD ; KIT
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31800713] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2021442]
项目资助者	National Natural Science Foundation of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation ; Physics
WOS类目	Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation ; Physics, Applied
WOS记录号	WOS:000881273400001
出版者	MDPI
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/130189
专题	中国科学院合肥物质科学研究院
通讯作者	Zhu, Ling; Yang, Ke
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Inst Opt & Fine Mech, Hefei 230031, Peoples R China 2.Anhui Med Univ, Sch Biomed Engn, Hefei 230032, Peoples R China
推荐引用方式 GB/T 7714	Yang, Xiao,Gao, Chaoru,Liu, Yong,et al. Simplified Cell Magnetic Isolation Assisted SC2 Chip to Realize "Sample in and Chemotaxis Out": Validated by Healthy and T2DM Patients' Neutrophils[J]. MICROMACHINES,2022,13.
APA	Yang, Xiao,Gao, Chaoru,Liu, Yong,Zhu, Ling,&Yang, Ke.(2022).Simplified Cell Magnetic Isolation Assisted SC2 Chip to Realize "Sample in and Chemotaxis Out": Validated by Healthy and T2DM Patients' Neutrophils.MICROMACHINES,13.
MLA	Yang, Xiao,et al."Simplified Cell Magnetic Isolation Assisted SC2 Chip to Realize "Sample in and Chemotaxis Out": Validated by Healthy and T2DM Patients' Neutrophils".MICROMACHINES 13(2022).