Picomolar thrombin detection by orchestration of triple signal amplification strategy with hierarchically porous Ti3C2Tx MXene electrode material-catalytic hairpin assembly reaction-metallic nanoprobes

doi:10.1016/j.bios.2022.114228

HFCAS OpenIR

	Picomolar thrombin detection by orchestration of triple signal amplification strategy with hierarchically porous Ti3C2Tx MXene electrode material-catalytic hairpin assembly reaction-metallic nanoprobes
	Yang, Pengqi 1,2; Guo, Xinyue 1,2; Zhang, Jia 1,3; Chen, Chaowen 1,3; Gan, Yuehao 1,2; Xie, Wenteng 1,2; Du, Yue 1,3; Wu, Zhengyan1,3
	2022-07-15
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
通讯作者	Zhang, Jia(zhangj@iim.ac.cn) ; Wu, Zhengyan(zywu@ipp.ac.cn)
摘要	Signal amplification strategies are essential to boost the sensitivity of detecting targeted ions or molecules with important biological functions, while few studies take advantage of signal amplification strategies more than two. As a "proof-of-concept" demonstration, we present the ultrasensitive electrochemical aptasensor for picomolar thrombin detection by synchronous coordination of triple signal amplification strategy. The porous MXene framework (PMXF) with secondary pores is constructed as carrier to increase electrons transfer channels, and thionine (as redox indicator) labelled Au nanorod (AuNR) and hollow Cu-Pt alloy (HCuPtA) are synthesized as the electrical signal amplifiers to enhance the response signals. In the presence of picomolar-level thrombin, catalytic hairpin assembly reactions of DNA are triggered to bridge thionine labelled AuNR or HCuPtA nanoprobes on the PMXF with controllablly scondary pore structures. Under the optimal conditionals, the sandwichtyped aptasensor based on PMXF-5/AuNR shows a more low limit of detection (LOD) of 0.67 pM with a linear range from 2 pM to 10 nM, while PMXF-5/HcuPtA exhibits a more wide linear range from 50 pM to 50 nM with a LOD of 16.67 pM for thormbin. This sensing platform can be customized to analyze other biological or environmental substances at an ultrahigh level by rationally designing DNA sequences of target-binding aptamer.
关键词	Hierarchically porous MXene framework Metallic nanoprobes Synergistic amplification strategy Electrochemical aptasensor Thrombin detection
DOI	10.1016/j.bios.2022.114228
关键词[WOS]	HIGH-PERFORMANCE ; LABEL-FREE ; APTASENSOR ; FABRICATION ; TI2CTX
收录类别	SCI
语种	英语
资助项目	University Synergy Innovation Program of Anhui Province[GXXT-2021-059] ; Special Project of Central Government for Local Science and Technology Development of Shandong Province[YDZX20203700001291] ; Open Research Fund of Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province
项目资助者	University Synergy Innovation Program of Anhui Province ; Special Project of Central Government for Local Science and Technology Development of Shandong Province ; Open Research Fund of Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号	WOS:000792492900004
出版者	ELSEVIER ADVANCED TECHNOLOGY
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/130784
专题	中国科学院合肥物质科学研究院
通讯作者	Zhang, Jia; Wu, Zhengyan
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Yang, Pengqi,Guo, Xinyue,Zhang, Jia,et al. Picomolar thrombin detection by orchestration of triple signal amplification strategy with hierarchically porous Ti3C2Tx MXene electrode material-catalytic hairpin assembly reaction-metallic nanoprobes[J]. BIOSENSORS & BIOELECTRONICS,2022,208.
APA	Yang, Pengqi.,Guo, Xinyue.,Zhang, Jia.,Chen, Chaowen.,Gan, Yuehao.,...&Wu, Zhengyan.(2022).Picomolar thrombin detection by orchestration of triple signal amplification strategy with hierarchically porous Ti3C2Tx MXene electrode material-catalytic hairpin assembly reaction-metallic nanoprobes.BIOSENSORS & BIOELECTRONICS,208.
MLA	Yang, Pengqi,et al."Picomolar thrombin detection by orchestration of triple signal amplification strategy with hierarchically porous Ti3C2Tx MXene electrode material-catalytic hairpin assembly reaction-metallic nanoprobes".BIOSENSORS & BIOELECTRONICS 208(2022).