Label-Free Detection and Translocation Dynamics Study of Single-Molecule Herceptin Using Solid-State Nanopores

doi:10.1002/admt.202200018

HFCAS OpenIR

	Label-Free Detection and Translocation Dynamics Study of Single-Molecule Herceptin Using Solid-State Nanopores
	Hu, Rui 1,2; Lu, Wenlong 1,2; Wei, Guanghao 1,2; Nan, Hexin 3; Li, Juan 3; Zhao, Qing 1,2,4
	2022-05-31
发表期刊	ADVANCED MATERIALS TECHNOLOGIES
ISSN	2365-709X
通讯作者	Hu, Rui(hurui1991@live.cn) ; Zhao, Qing(zhaoqing@pku.edu.cn)
摘要	Herceptin (or trastuzumab) is an important therapeutic monoclonal antibody (mAb) used in the treatment of HER2-positive breast cancer. Real-time counting and characterization of Herceptin is a fundamental step in the field of disease-related diagnosis and therapy. Solid-state nanopore-based biosensors have been proved to hold great potential in characterizing the properties of proteins at the single-molecule level for in vitro diagnosis. Here, the label-free detection and detailed translocation dynamics study of Herceptin using solid-state nanopores are demonstrated. By constricting nanopore size close to the size of Herceptin, the detection sensitivity and temporal resolution have been significantly improved, allowing the delicate probing of the structural information of single-molecule Herceptin. Therefore, three types of Herceptin translocation events are identified through nanopores, single-level, multi-level and spike-like events, emerged at different voltages regimes, indicating the unfolding kinetics of Herceptin under electric field. The potential influence of a high electric field on complex biomolecules is highlighted and a novel prospective platform is provided for label-free detection of single-molecule therapeutic monoclonal antibodies via solid-state nanopores as a miniaturized biomedical device.
关键词	solid-state nanopore single-molecule detection protein dynamics monoclonal antibody protein unfolding
DOI	10.1002/admt.202200018
关键词[WOS]	METASTATIC BREAST-CANCER ; MONOCLONAL-ANTIBODY ; PROTEINS ; TRASTUZUMAB ; IMMUNOGLOBULIN ; CHEMOTHERAPY ; FLEXIBILITY ; VINORELBINE ; BANDWIDTH ; ELISA
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2019YFA0707003] ; National Key Research and Development Program of China[2019YFE0114100] ; National Key Research and Development Program of China[2020YFA0909000] ; National Natural Science Foundation of China[NSFC 51872007] ; National Natural Science Foundation of China[NSFC 52103296] ; National Natural Science Foundation of China[NSFC 91959102] ; Joint Laboratory of Offshore Wind Power and Intelligent Energy System[HNKJ20-H88] ; Beijing Municipal Natural Science Foundation[7202094] ; Shanghai Rising-Star Program[19QA1405400]
项目资助者	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Joint Laboratory of Offshore Wind Power and Intelligent Energy System ; Beijing Municipal Natural Science Foundation ; Shanghai Rising-Star Program
WOS研究方向	Materials Science
WOS类目	Materials Science, Multidisciplinary
WOS记录号	WOS:000803014100001
出版者	WILEY
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/131334
专题	中国科学院合肥物质科学研究院
通讯作者	Hu, Rui; Zhao, Qing
作者单位	1.Peking Univ, Sch Phys, State Key Lab Mesoscop Phys, Beijing 100871, Peoples R China 2.Peking Univ, Sch Phys, Frontiers Sci Ctr Nanooptoelect, Beijing 100871, Peoples R China 3.Chinese Acad Sci, Univ Chinese Acad Sci, Canc Hosp, Zhejiang Canc Hosp,Inst Basic Med & Canc IBMC, Hangzhou 310022, Zhejiang, Peoples R China 4.Peking Univ, Yangtze Delta Inst Optoelect, Nantong 226010, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Hu, Rui,Lu, Wenlong,Wei, Guanghao,et al. Label-Free Detection and Translocation Dynamics Study of Single-Molecule Herceptin Using Solid-State Nanopores[J]. ADVANCED MATERIALS TECHNOLOGIES,2022.
APA	Hu, Rui,Lu, Wenlong,Wei, Guanghao,Nan, Hexin,Li, Juan,&Zhao, Qing.(2022).Label-Free Detection and Translocation Dynamics Study of Single-Molecule Herceptin Using Solid-State Nanopores.ADVANCED MATERIALS TECHNOLOGIES.
MLA	Hu, Rui,et al."Label-Free Detection and Translocation Dynamics Study of Single-Molecule Herceptin Using Solid-State Nanopores".ADVANCED MATERIALS TECHNOLOGIES (2022).