Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice

doi:10.3390/antiox11081457

HFCAS OpenIR

	Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice
	Yang, Miaomiao 1,2,3; Fan, Qiang 1,2; Hei, Tom K.4; Chen, Guodong 1; Cao, Wei 1,2; Meng, Gang3 ; Han, Wei1,5,6
	2022-08-01
发表期刊	ANTIOXIDANTS
通讯作者	Han, Wei(hanw@hfcas.ac.cn)
摘要	Radiation-induced lung injury (RILI), especially radiation pneumonitis (RP), is a common clinical complication associated with thoracic radiotherapy for malignant tumors. However, the specific contributions of each cell subtype to this process are unknown. Here, we provide the single-cell pathology landscape of the RP in a mouse model by unbiased single-cell RNA-seq (scRNA-seq). We found a decline of type 2 alveolar cells in the RP lung tissue, with an expansion of macrophages, especially the Fabp4low and Spp1high subgroup, while Fabp4high macrophages were almost depleted. We observed an elevated expression of multiple mitochondrial genes in the RP group, indicating a type 2 alveolar cell (AT2) response to oxidative stress. We also calculated the enrichment of a cGAS-STING signaling pathway, which may be involved in regulating inflammatory responses and cancer progression in AT2 cells of PR mice. We delineate markers and transcriptional states, identify a type 2 alveolar cell, and uncover fundamental determinants of lung fibrosis and inflammatory response in RP lung tissue of mice.
关键词	radiation pneumonitis (RP) single-cell RNA-seq (scRNA-seq) mouse oxidative stress
DOI	10.3390/antiox11081457
关键词[WOS]	LUNG ENDOTHELIAL-CELLS ; PULMONARY-FIBROSIS ; INJURY ; EXPRESSION ; CANCER
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81974484] ; National Natural Science Foundation of China[U20A20372] ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2019HSC-CIP010] ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection
项目资助者	National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Food Science & Technology
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Food Science & Technology
WOS记录号	WOS:000846315000001
出版者	MDPI
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/131815
专题	中国科学院合肥物质科学研究院
通讯作者	Han, Wei
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Inst Hlth & Med Technol, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China 3.Anhui Med Univ, Clin Pathol Ctr, Affiliated Hosp 4, Hefei 230022, Peoples R China 4.Columbia Univ, Coll Phys & Surg, Ctr Radiol Res, Med Ctr, New York, NY 10032 USA 5.Soochow Univ, Collaborat Innovat Ctr Radiat Med Jiangsu Higher, Suzhou 215123, Peoples R China 6.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou 215123, Peoples R China
推荐引用方式 GB/T 7714	Yang, Miaomiao,Fan, Qiang,Hei, Tom K.,et al. Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice[J]. ANTIOXIDANTS,2022,11.
APA	Yang, Miaomiao.,Fan, Qiang.,Hei, Tom K..,Chen, Guodong.,Cao, Wei.,...&Han, Wei.(2022).Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice.ANTIOXIDANTS,11.
MLA	Yang, Miaomiao,et al."Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice".ANTIOXIDANTS 11(2022).