Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment

doi:10.1186/s12951-023-02109-5

HFCAS OpenIR

	Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment
	Qingxin, Song 1; Kai, Jiang 2; Dandan, Zheng 1; Linyu, Jin 3; Xiuyuan, Chen 1; Yubo, Feng 1; Kun, Wang 1; Yingchao, Han 1; Hao, Chen 1; Jie, Song 4,5; Zhi, Chen 1; Hongxing, Shen 1
	2023-09-28
发表期刊	JOURNAL OF NANOBIOTECHNOLOGY
通讯作者	Jie, Song(sjie@sjtu.edu.cn) ; Zhi, Chen(mcgrady923@126.com) ; Hongxing, Shen(shenhxgk@126.com)
摘要	The pathogenesis of intervertebral disc degeneration (IVDD) is attributed to metabolic dysregulation within the extracellular matrix and heightened apoptosis of nucleus pulposus cells (NPC). Therefore, a potential therapeutic strategy for managing IVDD involves the reestablishment of metabolic equilibrium within the extracellular matrix and the suppression of excessive myeloid cell apoptosis. The microRNA, miR-5590, displays marked differential expression in degenerative nucleus pulposus (NP) tissues and exerts a direct influence on the regulation of DDX5 expression. This, in turn, modulates mammalian target of rapamycin (mTOR) phosphorylation, thereby impacting autophagy and apoptosis. However, ensuring the smooth delivery of miRNA to a specific injury site poses a significant challenge. To address this issue, a multifunctional DNA hydrogel was developed and subsequently loaded with miR-5590 via spherical nucleic acids (SNAs) for the treatment of IVDD. The hydrogel, which exhibits versatility, has the potential to be administered through injection at the site of injury, resulting in a consistent and prolonged release of miR-5590. This leads to the creation of a genetic microenvironment within the NP, which triggers the onset of autophagy in NPCs and subsequently suppresses apoptosis. As a result, this process regulates the metabolic equilibrium within the extracellular matrix, thereby impeding the in vitro and in vivo progression of IVDD. The amalgamation of miRNAs and biomaterials offers a promising therapeutic strategy for the management of IVDD in clinical settings.
关键词	Intervertebral disc degeneration Gene therapy DNA hydrogel Autophagy Extracellular matrix
DOI	10.1186/s12951-023-02109-5
关键词[WOS]	NUCLEUS PULPOSUS ; SENESCENCE
收录类别	SCI
语种	英语
资助项目	We thank the Instrumental Analysis Center of Shanghai Jiao Tong University for materials testing.
项目资助者	We thank the Instrumental Analysis Center of Shanghai Jiao Tong University for materials testing.
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS类目	Biotechnology & Applied Microbiology ; Nanoscience & Nanotechnology
WOS记录号	WOS:001074913900001
出版者	BMC
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/132580
专题	中国科学院合肥物质科学研究院
通讯作者	Jie, Song; Zhi, Chen; Hongxing, Shen
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Dept Spine Surg, 160 Pujian Rd, Shanghai 200127, Peoples R China 2.Fudan Univ, Ear Nose & Throat Hosp, Dept Ophthalmol & Vis Sci, Shanghai Eye, Shanghai, Peoples R China 3.Shanghai Jiao Tong Univ, Ruijin Hosp, Shanghai Inst Traumatol & Orthoped, Dept Orthoped,Shanghai Key Lab Prevent & Treatment, Shanghai, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Dept Instrument Sci & Engn, Shanghai 200240, Peoples R China 5.Univ Chinese Acad Sci, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Qingxin, Song,Kai, Jiang,Dandan, Zheng,et al. Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment[J]. JOURNAL OF NANOBIOTECHNOLOGY,2023,21.
APA	Qingxin, Song.,Kai, Jiang.,Dandan, Zheng.,Linyu, Jin.,Xiuyuan, Chen.,...&Hongxing, Shen.(2023).Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment.JOURNAL OF NANOBIOTECHNOLOGY,21.
MLA	Qingxin, Song,et al."Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment".JOURNAL OF NANOBIOTECHNOLOGY 21(2023).