Institutional Repository of Chinese Acad Sci, High Field Magnet Lab,Hefei 230031, Anhui, Peoples R China
NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes | |
Yong Liu1,2; Jing Yang1![]() ![]() | |
2023-09-28 | |
发表期刊 | International Journal of Molecular Sciences
![]() |
ISSN | 1422-0067 |
通讯作者 | Wang, Junfeng(junfeng@hmfl.ac.cn) ; Li, Yunyan(yyli@hmfl.ac.cn) |
摘要 | In mitochondria, the major subunits of oxidative phosphorylation complexes are translated by the mitochondrial ribosome (mito-ribosome). The correct insertion and assembly of these subunits into the inner mitochondrial membrane (IMM) are facilitated by mitochondrial oxidase assembly protein 1 (Oxa1) during the translation process. This co-translational insertion process involves an association between the mito-ribosome and the C-terminus of Oxa1 (Oxa1-CTD) Nuclear magnetic resonance (NMR) methods were mainly used to investigate the structural characterization of yeast Oxa1-CTD and its mode of interaction with the E. coli 70S ribosome. Oxa1-CTD forms a transient-helical structure within the residues P342–Q385, which were reported to form an alpha-helix when combining with the ribosome. Two conserved contact sites that could interact with the ribosome were further identified. The first site was located on the very end of the N-terminus (V321–I327), and the second one encompassed a stretch of amino acid residues I348–Q370. Based on our discoveries and previous reports, a model has been proposed in which Oxa1-CTD interacts with ribosomes, accompanied by transient-to-stable transitions at the second contact site. These observations may enhance our understanding of the potential role of Oxa1-CTD in facilitating the assembly of oxidative phosphorylation complexes and provide insight into the structural characteristics of Oxa1-CTD. |
关键词 | Oxa1 insertase C-terminus ribosome interactions NMR structural transitions |
DOI | doi.org/10.3390/ijms241914657 |
URL | 查看原文 |
关键词[WOS] | PROTEIN ; BINDING ; INSERTION ; OXIDASE ; REGIONS ; FAMILY ; ALPHA ; C-13 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | We would like to express our gratitude to the High Magnetic Field Laboratory of the Chinese Academy of Sciences for generously providing us access to their NMR spectrometers. Additionally, we created the graphical abstract using BioRender.com. |
项目资助者 | We would like to express our gratitude to the High Magnetic Field Laboratory of the Chinese Academy of Sciences for generously providing us access to their NMR spectrometers. Additionally, we created the graphical abstract using BioRender.com. |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
WOS记录号 | WOS:001086042000001 |
出版者 | MDPI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/133241 |
专题 | 中科院强磁场科学中心 |
通讯作者 | Yong Liu; Yunyan li |
作者单位 | 1.High Magnetic Field Laboratory, Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China 2.Science Island Branch of Graduate School, University of Science and Technology of China, Hefei 230026, China 3.Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, China |
第一作者单位 | 中科院强磁场科学中心 |
通讯作者单位 | 中科院强磁场科学中心 |
推荐引用方式 GB/T 7714 | Yong Liu,Jing Yang,Maosen Ruan,et al. NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes[J]. International Journal of Molecular Sciences,2023,24. |
APA | Yong Liu,Jing Yang,Maosen Ruan,Huiqin Zhang,Junfeng Wang,&Yunyan li.(2023).NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes.International Journal of Molecular Sciences,24. |
MLA | Yong Liu,et al."NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes".International Journal of Molecular Sciences 24(2023). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论