Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma

doi:10.1002/ppap.201300035

	Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma
	Ke, Zhigang 1; Huang, Qing1,2
	2013-08-01
发表期刊	PLASMA PROCESSES AND POLYMERS
摘要	The effects of non-thermal plasma at the argon gas-solution interface on horseradish peroxidase (HRP), a classical heme-containing protein, were assessed quantitatively. Evidence from spectroscopic approaches showed that enzymatic activity together with the contents of heme and iron in HRP decreased dramatically and concomitantly upon plasma treatment. The plasma induced both long- and short-lived reactive species which inactivated HRP in the solution. In particular, hydrogen peroxide broke the heme prosthetic group into fluorescent products, mainly dipyrrols and their derivatives, while other factors such as hydroxyl radicals and UV radiation generated during radicals and UV radiation generated during plasma discharge accelerated this heme protein degradation process.
文章类型	Article
关键词	Horseradish Peroxidase Hydrogen Peroxide Non-thermal Plasma Optical Spectroscopy Radicals
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/ppap.201300035
关键词[WOS]	DIELECTRIC BARRIER DISCHARGE ; GAS-SOLUTION INTERFACE ; ATMOSPHERIC-PRESSURE PLASMA ; M-CHLOROPEROXYBENZOIC ACID ; HYDROGEN-PEROXIDE ; INDUCED RADIOLYSIS ; NONTHERMAL PLASMA ; AQUEOUS-SOLUTION ; OXYGEN ; WATER
收录类别	SCI
语种	英语
WOS研究方向	Physics ; Polymer Science
WOS类目	Physics, Applied ; Physics, Fluids & Plasmas ; Physics, Condensed Matter ; Polymer Science
WOS记录号	WOS:000327790000007
引用统计	被引频次：47[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/22198
专题	技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Ke, Zhigang,Huang, Qing. Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma[J]. PLASMA PROCESSES AND POLYMERS,2013,10(8):731-739.
APA	Ke, Zhigang,&Huang, Qing.(2013).Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma.PLASMA PROCESSES AND POLYMERS,10(8),731-739.
MLA	Ke, Zhigang,et al."Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma".PLASMA PROCESSES AND POLYMERS 10.8(2013):731-739.