Institutional Repository of Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, People's Republic of China
Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma | |
Ke, Zhigang1; Huang, Qing1,2 | |
2013-08-01 | |
发表期刊 | PLASMA PROCESSES AND POLYMERS |
摘要 | The effects of non-thermal plasma at the argon gas-solution interface on horseradish peroxidase (HRP), a classical heme-containing protein, were assessed quantitatively. Evidence from spectroscopic approaches showed that enzymatic activity together with the contents of heme and iron in HRP decreased dramatically and concomitantly upon plasma treatment. The plasma induced both long- and short-lived reactive species which inactivated HRP in the solution. In particular, hydrogen peroxide broke the heme prosthetic group into fluorescent products, mainly dipyrrols and their derivatives, while other factors such as hydroxyl radicals and UV radiation generated during radicals and UV radiation generated during plasma discharge accelerated this heme protein degradation process. |
文章类型 | Article |
关键词 | Horseradish Peroxidase Hydrogen Peroxide Non-thermal Plasma Optical Spectroscopy Radicals |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1002/ppap.201300035 |
关键词[WOS] | DIELECTRIC BARRIER DISCHARGE ; GAS-SOLUTION INTERFACE ; ATMOSPHERIC-PRESSURE PLASMA ; M-CHLOROPEROXYBENZOIC ACID ; HYDROGEN-PEROXIDE ; INDUCED RADIOLYSIS ; NONTHERMAL PLASMA ; AQUEOUS-SOLUTION ; OXYGEN ; WATER |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Physics ; Polymer Science |
WOS类目 | Physics, Applied ; Physics, Fluids & Plasmas ; Physics, Condensed Matter ; Polymer Science |
WOS记录号 | WOS:000327790000007 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/22198 |
专题 | 技术生物与农业工程研究所 |
作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China |
推荐引用方式 GB/T 7714 | Ke, Zhigang,Huang, Qing. Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma[J]. PLASMA PROCESSES AND POLYMERS,2013,10(8):731-739. |
APA | Ke, Zhigang,&Huang, Qing.(2013).Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma.PLASMA PROCESSES AND POLYMERS,10(8),731-739. |
MLA | Ke, Zhigang,et al."Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma".PLASMA PROCESSES AND POLYMERS 10.8(2013):731-739. |
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