Institutional Repository of Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, People's Republic of China
| Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb. | |
Hou, Jinyan1 ; Wu, Ying1,2,3; Shen, Yangcheng1; Mao, Yingji1,2; Liu, Wenbo1,2; Zhao, Weiwei1; Mu, Yan1; Li, Minghao1; Yang, Minglei1; Wu, Lifang1,2
| |
| 2015-12-14 | |
| 发表期刊 | SCIENTIA HORTICULTURAE
 |
| 摘要 | Sapium sebiferium Roxb. is a highly valued ornamental plant species in tropical and subtropical regions. Here we present an efficient protocol for plant regeneration from immature zygotic embryos (IZEs) of S. sebiferium through direct somatic embryogenesis and shoot organogenesis for the first time. The results showed that mechanical damage, plant growth regulators (PGRs) and light regimes had great influence on in vitro morphogenesis. High frequency of somatic embryogenesis was obtained when the mechanical damaged IZEs were cultured on the Murashige and Skoog (MS) medium containing 1.0 mgl(-1) indole-3-acetic acid (IAA) under continuous darkness for two weeks followed by a light/dark photoperiod cycle for four weeks. The induction frequency of somatic embryogenesis was up to 90.0%. Somatic embryos (SEs) at early cotyledonary stage showed highest proliferation frequency (81.7%) and 80.0% of secondary somatic embryos (SSEs) germinated on plant growth regulators (PGRs)-free MS medium. For direct shoot induction pathway, the best shoot regeneration rate (63.3%) was obtained on the medium supplemented with 1.0 mgl-1 6-benzyladenine (6-BA). The plantlets derived from two ways were successfully acclimatized in the greenhouse with more than 90% survival rate. This study will be beneficial for the rescue of immature embryo, inheritance of heterosis as well as the genetic improvement of S. sebiferium. (C) 2015 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Chinese Tallow Tree Somatic Embryogenesis Mechanical Damage Secondary Somatic Embryos Adventitious Shoots Plant Regeneration |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.scienta.2015.09.040 |
| 关键词[WOS] | CHINESE TALLOW TREE ; MEDICINAL-PLANT ; TISSUE-CULTURE ; L. ; GROWTH ; BALDCYPRESS ; INITIATION ; INDUCTION ; HYPOCOTYL ; LEAF |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; National Nature Science Foundation of China(11375232) ; National Nature Science Foundation of China(11375232) ; National Nature Science Foundation of China(11375232) ; National Nature Science Foundation of China(11375232) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) |
| WOS研究方向 | Agriculture |
| WOS类目 | Horticulture |
| WOS记录号 | WOS:000367411400028 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/22210 |
| 专题 | 技术生物与农业工程研究所 |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn & Bioenergy, Forest Res Ctr,State Forestry Adm, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China 3.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471023, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Jinyan,Wu, Ying,Shen, Yangcheng,et al. Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb.[J]. SCIENTIA HORTICULTURAE,2015,197(无):218-225. |
| APA | Hou, Jinyan.,Wu, Ying.,Shen, Yangcheng.,Mao, Yingji.,Liu, Wenbo.,...&Wu, Lifang.(2015).Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb..SCIENTIA HORTICULTURAE,197(无),218-225. |
| MLA | Hou, Jinyan,et al."Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb.".SCIENTIA HORTICULTURAE 197.无(2015):218-225. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Plant regeneration t（2826KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论