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Structure of an E. coli integral membrane sulfurtransferase and its structural transition upon SCN- binding defined by EPR-based hybrid method
Ling, Shenglong1,2; Wang, Wei1,2; Yu, Lu1,2; Peng, Junhui1,2; Cai, Xiaoying1,2; Xiong, Ying1,2; Hayati, Zahra3; Zhang, Longhua1,2; Zhang, Zhiyong1,2; Song, Likai3; Tian, Changlin1,2
2016-01-28
Source PublicationSCIENTIFIC REPORTS
Volume6Issue:Pages:1-12
AbstractElectron paramagnetic resonance (EPR)-based hybrid experimental and computational approaches were applied to determine the structure of a full-length E. coli integral membrane sulfurtransferase, dimeric YgaP, and its structural and dynamic changes upon ligand binding. The solution NMR structures of the YgaP transmembrane domain (TMD) and cytosolic catalytic rhodanese domain were reported recently, but the tertiary fold of full-length YgaP was not yet available. Here, systematic site-specific EPR analysis defined a helix-loop-helix secondary structure of the YagP-TMD monomers using mobility, accessibility and membrane immersion measurements. The tertiary folds of dimeric YgaP-TMD and full-length YgaP in detergent micelles were determined through inter-and intra-monomer distance mapping and rigid-body computation. Further EPR analysis demonstrated the tight packing of the two YgaP second transmembrane helices upon binding of the catalytic product SCN-, which provides insight into the thiocyanate exportation mechanism of YgaP in the E. coli membrane.
SubtypeArticle
WOS HeadingsScience & Technology
Funding OrganizationNational Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176)
DOI10.1038/srep20025
WOS KeywordPROTEIN-STRUCTURE DETERMINATION ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; CONFORMATIONAL DYNAMICS ; SPIN LABELS ; SIDE-CHAINS ; CELL-CYCLE ; CW-EPR ; DOMAIN ; RHODANESE
Indexed BySCI
Language英语
Funding OrganizationNational Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; U.S. National High Magnetic Field Laboratory (NHFML)(5080) ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; State of Florida ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; U.S. National Science Foundation(DMR-1157490) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176) ; 21473176)
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000368783700001
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/22236
Collection中科院强磁场科学中心
Affiliation1.Univ Sci & Technol China, Natl Lab Phys Sci Microscale, Sch Life Sci, Hefei 230027, Peoples R China
2.Chinese Acad Sci, High Field Magnet Lab, Hefei 230027, Peoples R China
3.Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32310 USA
First Author AffilicationChinese Acad Sci, High Field Magnet Lab,Hefei 230031, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Ling, Shenglong,Wang, Wei,Yu, Lu,et al. Structure of an E. coli integral membrane sulfurtransferase and its structural transition upon SCN- binding defined by EPR-based hybrid method[J]. SCIENTIFIC REPORTS,2016,6(无):1-12.
APA Ling, Shenglong.,Wang, Wei.,Yu, Lu.,Peng, Junhui.,Cai, Xiaoying.,...&Tian, Changlin.(2016).Structure of an E. coli integral membrane sulfurtransferase and its structural transition upon SCN- binding defined by EPR-based hybrid method.SCIENTIFIC REPORTS,6(无),1-12.
MLA Ling, Shenglong,et al."Structure of an E. coli integral membrane sulfurtransferase and its structural transition upon SCN- binding defined by EPR-based hybrid method".SCIENTIFIC REPORTS 6.无(2016):1-12.
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