Institutional Repository of Chinese Acad Sci, High Field Magnet Lab,Hefei 230031, Anhui, Peoples R China
| Using "On/Off" F-19 NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates | |
Zheng, Zhen1; Sun, Hongbin2; Hu, Chen2 ; Li, Gongyu1; Liu, Xiaomei1; Chen, Peiyao1; Cui, Yusi1; Liu, Jing2; Wang, Junfeng2; Liang, Gaolin1
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| 2016-03-15 | |
| 发表期刊 | ANALYTICAL CHEMISTRY
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| 摘要 | Tyrosine kinase and phosphatase are two important, antagonistic enzymes in organisms. Development of noninvasive approach for sensing their activity with high spatial and temporal resolution remains challenging. Herein, we rationally designed a hydrogelator Nap-Phe-Phe(CF3)-Glu-Tyr-Ile-OH (1a) whose supramolecular hydrogel (i.e., Gel 1a) can be subjected to tyrosine kinase-directed disassembly, and its phosphate precursor Nap-Phe-Phe(CF3)-Glu-Tyr(H2PO3)-Ile-OH (1b) which: can be subjected to alkaline phosphatase (ALP)-instructed self-assembly to fonn supramolecular hydro gel Gel 1b, respectively.. Mechanic properties and internal fibrous networks of the hydrogels were characterized with rheology and cryo transmission electron microscopy (cryo-TEM). Disassembly/Self-asSemblY of their corresponding supramolecular hydrogels conferring respective "On/Off' F-19 NMR/MRI signals were employed to sense the activity of these two important enzymes in vitro and in cell lysates for the first time. We anticipate that our new F-19 NMR/magnetic, resonance imaging (MRI) method would facilitate pharmaceutical researchers to screen new inhibitors for these two enzymes without steric hindrance. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1021/acs.analchem.6b00036 |
| 关键词[WOS] | PROTEIN-KINASE ACTIVITY ; ENZYMATIC HYDROGELATION ; PHOSPHATASE-ACTIVITIES ; NANOPARTICLES ; PHOSPHORYLATION ; NANOPROBES ; PROBES ; SWITCH ; VIVO ; MRI |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000372391500053 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/22340 |
| 专题 | 中科院强磁场科学中心 |
| 作者单位 | 1.Univ Sci & Technol China, Dept Chem, Hefei Sci Ctr CAS, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Zhen,Sun, Hongbin,Hu, Chen,et al. Using "On/Off" F-19 NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates[J]. ANALYTICAL CHEMISTRY,2016,88(6):3363-3368. |
| APA | Zheng, Zhen.,Sun, Hongbin.,Hu, Chen.,Li, Gongyu.,Liu, Xiaomei.,...&Liang, Gaolin.(2016).Using "On/Off" F-19 NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates.ANALYTICAL CHEMISTRY,88(6),3363-3368. |
| MLA | Zheng, Zhen,et al."Using "On/Off" F-19 NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates".ANALYTICAL CHEMISTRY 88.6(2016):3363-3368. |
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