Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy

doi:10.1021/acs.analchem.6b00400

	Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy
	Zhang, Fengqiu 1,2,3; Huang, Qing 1,2; Yan, Jingwen 1; Chen, Zhu 1
	2016-04-19
发表期刊	ANALYTICAL CHEMISTRY
摘要	A nucleosome is made up of DNA and histones, and acetylation of histones perturbs the interaction of DNA and histones and thus affects the chromatin conformation and function. However, whether or how acetylation induces DNA conformation changes is still elusive. In this work, we applied FT-IR spectroscopy to monitor the DNA signals in cells as the histone acetylation was regulated by trichostatin A (TSA), a reversible inhibitor to histone deacetylases (HDACs). Our results unambiguously demonstrate the significant transformation of B-DNA to Z-DNA upon histone acetylation in the TSA treated HeLa cells: This is the first report providing the explicit experimental evidence for such a B-Z transformation of DNA in the epigenetic states of cells.
文章类型	Article
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1021/acs.analchem.6b00400
关键词[WOS]	LINKING NUMBER CHANGE ; TRICHOSTATIN-A ; INFRARED MICROSPECTROSCOPY ; DEACETYLASE INHIBITORS ; CHROMATIN STRUCTURE ; NUCLEIC-ACIDS ; DIFFERENTIATION ; CANCER ; THERAPY ; ACETYLTRANSFERASES
收录类别	SCI
语种	英语
项目资助者	Major National Scientific Research Project(2014CB932002 ; Major National Scientific Research Project(2014CB932002 ; Major National Scientific Research Project(2014CB932002 ; Major National Scientific Research Project(2014CB932002 ; National Nature Science Foundation of China(11475217) ; National Nature Science Foundation of China(11475217) ; National Nature Science Foundation of China(11475217) ; National Nature Science Foundation of China(11475217) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; 2013CB934304) ; 2013CB934304) ; 2013CB934304) ; 2013CB934304)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000374706000003
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/22396
专题	技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Key Lab Ion Beam Bioengn, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Hefei, Peoples R China 2.Univ Sci & Technol China, Sch Nucl Sci & Technol, Hefei 230026, Peoples R China 3.Zhengzhou Univ, Sch Phys Engn, Henan Key Lab Ion Beam Bioengn, Zhengzhou 450052, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Fengqiu,Huang, Qing,Yan, Jingwen,et al. Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy[J]. ANALYTICAL CHEMISTRY,2016,88(8):4179-4182.
APA	Zhang, Fengqiu,Huang, Qing,Yan, Jingwen,&Chen, Zhu.(2016).Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy.ANALYTICAL CHEMISTRY,88(8),4179-4182.
MLA	Zhang, Fengqiu,et al."Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy".ANALYTICAL CHEMISTRY 88.8(2016):4179-4182.

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