Institutional Repository of Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, People's Republic of China
| Investigating the environmental factors affecting the toxicity of silver nanoparticles in Escherichia coli with dual fluorescence analysis | |
Hong, Wei1,2; Li, Luzhi1,2; Liang, Junting1,2; Wang, Jingjing1,2 ; Wang, Xuanyu1,2; Xu, Shengmin1,2; Wu, Lijun1,2; Zhao, Guoping1,2; Xu, An1,2; Chen, Shaopeng1,2
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| 2016-07-01 | |
| 发表期刊 | CHEMOSPHERE
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| 摘要 | Flow cytometric investigation of the toxic effects of nanoparticles on bacteria is highly challenging and not sensitive due to the interference of aggregated nanoparticles: aggregated nanoparticles and bacteria are similar in size. In this study, an optimized dual fluorescence flow cytometric analysis was developed using PI-Lac::GFP (propidium iodide stained Escherichia coli (lac::GFP)) to monitor the toxicity of silver nanoparticles (AgNPs). As compared with single fluorescence analysis, the dual fluorescence analysis enabled more accurate evaluation of the toxic effects of AgNPs. We used this dual fluorescence analysis to investigate how AgNPs toxicity was affected by two typical environmental factors, divalent metal ions and surfactants. Our data revealed that Cu2+ and SDS significantly enhanced the toxicity of AgNPs in a dose-dependent manner. SDS enhanced the toxicity of both AgNPs and Ag+ ions, whereas Cu2+ increased the toxicity of AgNPs but not dissolved Ag+ ions. Our results suggest that this dual fluorescence analysis can be used to evaluate the toxicity of AgNPs accurately and sensitively. (C) 2016 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Silver Nanoparticles Toxic Effects Dual Fluorescence Analysis Environmental Factor |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.chemosphere.2016.04.074 |
| 关键词[WOS] | ANTIBACTERIAL ACTIVITY ; FLOW-CYTOMETRY ; E. COLI ; STABILITY ; MECHANISM ; AGGREGATION ; DISSOLUTION ; INHIBITION ; PARTICLES ; SURFACES |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams |
| WOS研究方向 | Environmental Sciences & Ecology |
| WOS类目 | Environmental Sciences |
| WOS记录号 | WOS:000377736100039 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/22455 |
| 专题 | 技术生物与农业工程研究所 |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei, Anhui, Peoples R China 2.Key Lab Environm Toxicol & Pollut Control Technol, Hefei, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hong, Wei,Li, Luzhi,Liang, Junting,et al. Investigating the environmental factors affecting the toxicity of silver nanoparticles in Escherichia coli with dual fluorescence analysis[J]. CHEMOSPHERE,2016,155(无):329-335. |
| APA | Hong, Wei.,Li, Luzhi.,Liang, Junting.,Wang, Jingjing.,Wang, Xuanyu.,...&Chen, Shaopeng.(2016).Investigating the environmental factors affecting the toxicity of silver nanoparticles in Escherichia coli with dual fluorescence analysis.CHEMOSPHERE,155(无),329-335. |
| MLA | Hong, Wei,et al."Investigating the environmental factors affecting the toxicity of silver nanoparticles in Escherichia coli with dual fluorescence analysis".CHEMOSPHERE 155.无(2016):329-335. |
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