Institutional Repository of Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, People's Republic of China
| A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide | |
Ke, Zhigang1; Ma, Shanshan1; Li, Lamei1; Huang, Qing1,2
| |
| 2016-07-16 | |
| 发表期刊 | CHEMICAL PHYSICS LETTERS
 |
| 摘要 | Horseradish peroxidase (HRP) is a classical heme-containing protein which has been applied in many fields. The prosthetic group heme in HRP, especially in unfolded state, can react with hydrogen peroxide (H2O2) to produce a fluorescent product with the maximum emission wavelength at 450 nm. Utilizing this emission band as a fluorescence probe, the unfolding process of HRP in urea can be assessed quantitatively, and the calculated thermodynamic parameters are consistent with those determined by circular dichroism (CD) at 222 nm and steady-state tryptophan (Trp) fluorescence methods. (C) 2016 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Fluorescence Horseradish Peroxidase Protein Unfolding Heme |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1016/j.cplett.2016.05.055 |
| 关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; X-RAY-SCATTERING ; CIRCULAR-DICHROISM ; GUANIDINE-HYDROCHLORIDE ; CYTOCHROME-C ; PROTEINS ; DENATURATION ; SPECTROSCOPY ; INACTIVATION ; STABILITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; 11175204 ; 11175204 ; 11175204 ; 11175204 ; 11475217 ; 11475217 ; 11475217 ; 11475217 ; 11405220) ; 11405220) ; 11405220) ; 11405220) |
| WOS研究方向 | Chemistry ; Physics |
| WOS类目 | Chemistry, Physical ; Physics, Atomic, Molecular & Chemical |
| WOS记录号 | WOS:000383444700009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/24255 |
| 专题 | 技术生物与农业工程研究所 |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230026, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ke, Zhigang,Ma, Shanshan,Li, Lamei,et al. A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide[J]. CHEMICAL PHYSICS LETTERS,2016,657(无):49-52. |
| APA | Ke, Zhigang,Ma, Shanshan,Li, Lamei,&Huang, Qing.(2016).A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide.CHEMICAL PHYSICS LETTERS,657(无),49-52. |
| MLA | Ke, Zhigang,et al."A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide".CHEMICAL PHYSICS LETTERS 657.无(2016):49-52. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A fluorescence appro（620KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论