Institutional Repository of Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
| Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe | |
Zhang, Ruilong1,2; Zhao, Jun1; Han, Guangmei1; Liu, Zhengjie1,2; Liu, Cui1,2; Zhang, Cheng1,2; Liu, Bianhua1; Jiang, Changlong1; Liu, Renyong1; Zhao, Tingting1; Han, Ming-Yong1,3; Zhang, Zhongping1,2,4
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| 2016-03-23 | |
| 发表期刊 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
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| 摘要 | Fluorescent probes are powerful tools for the investigations of reactive oxygen species (ROS) in living organisms by visualization and imaging. However, the multiparallel assays of several ROS with multiple probes are often limited by the available number of spectrally nonoverlapping chromophores together with large invasive effects and discrepant biological locations. Meanwhile, the spontaneous ROS profilings in various living organs/tissues are also limited by the penetration capability of probes across different biological barriers and the stability in reactive in vivo environments. Here, we report a single fluorescent probe to achieve the effective discrimination and profiling of hydroxyl radicals (center dot OH) and hypochlorous acid (HClO) in living organisms. The probe is constructed by chemically grafting an additional five-membered heterocyclic ring and a lateral triethylene glycol chain to a fluorescein mother, which does not only turn off the fluorescence of fluorescein, but also create the dual reactive sites to ROS and the penetration capability in passing through various biological barriers. The reactions of probe with center dot OH and HClO simultaneously result in cyan and green emissions, respectively, providing the real-time discrimination and quantitative analysis of the two ROS in cellular mitochondria. Surprisingly, the accumulation of probes in the intestine and liver of a normal-state zebrafish and the transfer pathway from intestine-to-blood-to-organ/tissue-to-kidney-to-excretion clearly present the profiling of spontaneous center dot OH and HClO in these metabolic organs. In particular, the stress generation of center dot OH at the fresh wound of zebrafish is successfully visualized for the first time, in spite of its extremely short lifetime. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1021/jacs.5b12848 |
| 关键词[WOS] | HYPOCHLOROUS ACID DETECTION ; HYDROGEN-PEROXIDE ; IN-VIVO ; OXIDATIVE STRESS ; FREE-RADICALS ; CELLS ; SUPEROXIDE ; MECHANISMS ; OXIDANTS ; DISEASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Basic Research Program of China(2015CB932002) ; National Basic Research Program of China(2015CB932002) ; National Basic Research Program of China(2015CB932002) ; National Basic Research Program of China(2015CB932002) ; China-Singapore Joint Project(2015DFG92510) ; China-Singapore Joint Project(2015DFG92510) ; China-Singapore Joint Project(2015DFG92510) ; China-Singapore Joint Project(2015DFG92510) ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 1508085SQB200 ; 1508085SQB200 ; 1508085SQB200 ; 1508085SQB200 ; 21375131 ; 21375131 ; 21375131 ; 21375131 ; 1608085QB32) ; 1608085QB32) ; 1608085QB32) ; 1608085QB32) ; 21275145 ; 21275145 ; 21275145 ; 21275145 ; 21371174) ; 21371174) ; 21371174) ; 21371174) |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| WOS记录号 | WOS:000372854200025 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/30903 |
| 专题 | 中科院合肥智能机械研究所 |
| 作者单位 | 1.Chinese Acad Sci, Ctr Excellence Nanosci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 3.ASTAR, Inst Mat Res & Engn, 3 Res Link, Singapore 117602, Singapore 4.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ruilong,Zhao, Jun,Han, Guangmei,et al. Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2016,138(11):3769-3778. |
| APA | Zhang, Ruilong.,Zhao, Jun.,Han, Guangmei.,Liu, Zhengjie.,Liu, Cui.,...&Zhang, Zhongping.(2016).Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,138(11),3769-3778. |
| MLA | Zhang, Ruilong,et al."Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 138.11(2016):3769-3778. |
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