Institutional Repository of Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
| Establishment of N-succinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) modified biochip enabling concurrent detection of serum infectious antibodies in neuroborreliosis | |
| Ye, Lei1; Huang, Na-Li1; Ma, Xue-Ling2; Schneider, Marion3; Huang, Xin-Jiu4; Du, Wei-Dong3,5 | |
| 2016-04-15 | |
| 发表期刊 | BIOSENSORS & BIOELECTRONICS
 |
| 摘要 | In this study, we developed a novel protein biochip that was modified with N-succinimidyl 4-(maleimidomethyl)cyclohexanecarboxylate (SMCC) and specialized for concurrent detection of serum IgG and 1gM antibodies against Borrelia burgdorferi antigens, flagellin, outer surface protein C (OspC) and variable major protein-like sequence (VIsE) in the patients with neuroborreliosis (NB), respectively. Surface chemical characteristics of the biochips were validated with atomic force microscope (AFM) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The visualized detection limit for IgG antibodies against flagellin, OspC and VIsE antigens on the biochip were 0.78 mu g/ml, 0.78, mu g/ml and 1.56 mu g/ml, respectively. Finally, serum IgG and IgM antibodies in 72 patients with NB and 188 healthy individuals were tested on the biochip. The seroimmunological outcome by the biochip were evaluated in comparison with enzyme linked immunosorbent assay (ELISA) assay. The results demonstrated that the prevalences of IgG and IgM antibodies in the cases were 41.7%, 63.9% to flagellin; 20.8% and 51.4% to OspC and 76.4%, 62.5% to VIsE, respectively. Utilization of the biochip in detection IgM antibody against flagellin was compatible with ELISA assay (R-2=0.849). Thus, the protein biochip would provide a potential platform not only for enabling detection of corresponding antibodies directed against B. burgdorferi antigens, but also for monitoring course of the disease. (C) 2015 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Protein Biochip N-succinimidyl 4-(Maleimidomethyl) Cyclohexanecarboxylate (Smcc) Flagellin Outer Surface Protein c (Ospc) Variable Major Protein-like Sequence (Vise) Neuroborreliosis Elisa |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.bios.2015.11.050 |
| 关键词[WOS] | BORRELIA-BURGDORFERI ; LYME BORRELIOSIS ; ERYTHEMA MIGRANS ; DISEASE ; DIAGNOSIS ; VALIDATION ; GOLD ; OSPC |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Natural Science Foundation of China(81171655) ; National Natural Science Foundation of China(81171655) ; National Natural Science Foundation of China(81171655) ; National Natural Science Foundation of China(81171655) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; International Science and Technology cooperation program of Anhui Province(1403062023) |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| WOS记录号 | WOS:000368954900057 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/31583 |
| 专题 | 中科院合肥智能机械研究所 |
| 作者单位 | 1.Anhui Med Univ, Sch Life Sci, Dept Biol, Meishan 81, Hefei 230032, Peoples R China 2.ACURA Kliniken, MVZ, D-76530 Baden Baden, Germany 3.Univ Ulm Klinikum, Sekt Expt Anasthesiol, Albert Einstein Allee 23, D-89081 Ulm, Germany 4.Chinese Acad Sci, Inst Intelligent Machines, Res Ctr Biomimet Funct Mat & Sensing Devices, Hefei 230031, Peoples R China 5.Anhui Med Univ, Dept Pathol, Meishan 81, Hefei 230032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Lei,Huang, Na-Li,Ma, Xue-Ling,et al. Establishment of N-succinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) modified biochip enabling concurrent detection of serum infectious antibodies in neuroborreliosis[J]. BIOSENSORS & BIOELECTRONICS,2016,78(无):404-410. |
| APA | Ye, Lei,Huang, Na-Li,Ma, Xue-Ling,Schneider, Marion,Huang, Xin-Jiu,&Du, Wei-Dong.(2016).Establishment of N-succinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) modified biochip enabling concurrent detection of serum infectious antibodies in neuroborreliosis.BIOSENSORS & BIOELECTRONICS,78(无),404-410. |
| MLA | Ye, Lei,et al."Establishment of N-succinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) modified biochip enabling concurrent detection of serum infectious antibodies in neuroborreliosis".BIOSENSORS & BIOELECTRONICS 78.无(2016):404-410. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Establishment of N-s（8136KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论