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Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices
Yang, Ke1,2; Peretz-Soroka, Hagit2; Wu, Jiandong2; Zhu, Ling1; Cui, Xueling3; Zhang, Michael4; Rigatto, Claudio4; Liu, Yong1; Lin, Francis2,5,6,7
2017-06-08
Source PublicationSCIENTIFIC REPORTS
Volume7Issue:Pages:1-12
AbstractNeutrophil trafficking in tissues critically regulates the body's immune response. Neutrophil migration can either play a protective role in host defense or cause health problems. Fibroblast growth factor 23 (FGF23) is a known biomarker for chronic kidney disease (CKD) and was recently shown to impair neutrophil arrest on endothelium and transendothelial migration. In the present study, we further examined the effect of FGF23 on human blood neutrophil chemotaxis using two new microfluidic devices. Our results showed that chemotaxis of FGF23 pre-treated neutrophils to a fMLP gradient, in the presence or absence of a uniform FGF23 background, is quantitatively lower compared to the control cells. This effect is accompanied with a stronger drifting of FGF23 pre-treated cells along the flow. However, without the FGF23 pre-treatment, the FGF23 background only reduces chemotaxis of transmigrated cells through the thin barrier channel to the fMLP gradient. The effect of FGF23 on neutrophil migration and the correlation between multiple cell migration parameters are further revealed by chemotactic entropy and principle component analysis. Collectively, these results revealed the effect of FGF23 on weakening neutrophil chemotaxis, which shed light on FGF23 mediated neutrophil migration with direct disease relevance such as CKD.
SubtypeArticle
WOS HeadingsScience & Technology
Funding OrganizationNatural Sciences and Engineering Research Council of Canada (NSERC) ; Canadian Institutes of Health Research (CIHR) ; CMC Microsystems ; Winnipeg Rh Institute Foundation ; University of Manitoba ; NSERC ; Mitacs ; Natural Sciences and Engineering Research Council of Canada (NSERC) ; Canadian Institutes of Health Research (CIHR) ; CMC Microsystems ; Winnipeg Rh Institute Foundation ; University of Manitoba ; NSERC ; Mitacs
DOI10.1038/s41598-017-03210-0
WOS KeywordCHRONIC KIDNEY-DISEASE ; FGF23 ; MORTALITY ; MIGRATION ; ACTIVATION ; INFECTION ; DIALYSIS ; IMMUNITY ; SEPSIS ; INNATE
Indexed BySCI
Language英语
Funding OrganizationNatural Sciences and Engineering Research Council of Canada (NSERC) ; Canadian Institutes of Health Research (CIHR) ; CMC Microsystems ; Winnipeg Rh Institute Foundation ; University of Manitoba ; NSERC ; Mitacs ; Natural Sciences and Engineering Research Council of Canada (NSERC) ; Canadian Institutes of Health Research (CIHR) ; CMC Microsystems ; Winnipeg Rh Institute Foundation ; University of Manitoba ; NSERC ; Mitacs
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000402865200012
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Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/31920
Collection应用技术研究所
Affiliation1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol, Hefei, Anhui, Peoples R China
2.Univ Manitoba, Dept Phys & Astron, Winnipeg, MB, Canada
3.Jilin Univ, Dept Genet, Changchun, Jilin Sheng, Peoples R China
4.Seven Oaks Gen Hosp, Winnipeg, MB, Canada
5.Univ Manitoba, Dept Biosyst Engn, Winnipeg, MB, Canada
6.Univ Manitoba, Dept Immunol, Winnipeg, MB, Canada
7.Univ Manitoba, Dept Biol Sci, Winnipeg, MB, Canada
Recommended Citation
GB/T 7714
Yang, Ke,Peretz-Soroka, Hagit,Wu, Jiandong,et al. Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices[J]. SCIENTIFIC REPORTS,2017,7(无):1-12.
APA Yang, Ke.,Peretz-Soroka, Hagit.,Wu, Jiandong.,Zhu, Ling.,Cui, Xueling.,...&Lin, Francis.(2017).Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices.SCIENTIFIC REPORTS,7(无),1-12.
MLA Yang, Ke,et al."Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices".SCIENTIFIC REPORTS 7.无(2017):1-12.
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