Institutional Repository of Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
| Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe | |
| Lei, Kepeng1,2; Sun, Mingtai1; Du, Libo5; Zhang, Xiaojie5; Yu, Huan1; Wang, Suhua1,2,3,4; Hayat, Tasawar3; Alsaedi, Ahmed3 | |
| 2017-08-01 | |
| 发表期刊 | TALANTA
 |
| 摘要 | The sensitive and selective fluorescence probe for hydroxyl radical analysis is of significance because hydroxyl radical plays key roles in many physiological and pathological processes. In this work, a novel organic fluorescence molecular probe OHP for hydroxyl radical is synthesized by a two-step route. The probe employs 4-bora-3a,4a-diaza-s-indacene (difluoroboron dipyrromethene, BODIPY) as the fluorophore and possesses relatively high fluorescence quantum yields (77.14%). Hydroxyl radical can rapidly react with the probe and quench the fluorescence in a good linear relationship (R-2=0.9967). The limit of detection is determined to be as low as 11 nM. In addition, it has been demonstrated that the probe has a good stability against pH and light illumination, low cytotoxicity and high biocompatibility. Cell culture experimental results show that the probe OHP is sensitive and selective for imaging and tracking endogenous hydroxyl radical in live cells. |
| 文章类型 | Article |
| 关键词 | Bodipy Cell Imaging Fluorescent Probe Hydroxyl Radical Sensitive And Selective |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1016/j.talanta.2017.04.004 |
| 关键词[WOS] | TURN-ON DETECTION ; QUANTUM DOTS ; LIVING CELLS ; SELECTIVE DETECTION ; HYPOCHLOROUS ACID ; RATIOMETRIC FLUORESCENCE ; GOLD NANOCLUSTERS ; OXIDATIVE STRESS ; NITROGEN-DIOXIDE ; OXYGEN RADICALS |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000402343200043 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/31957 |
| 专题 | 中科院合肥智能机械研究所 |
| 作者单位 | 1.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Dept Mat Sci & Engn, Hefei 230026, Anhui, Peoples R China 3.King Abdulaziz Univ, NAAM Res Grp, Fac Sci, Jeddah 21589, Saudi Arabia 4.North China Elect Power Univ, Sch Environm & Chem Engn, Beijing 102206, Peoples R China 5.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China |
| 第一作者单位 | 中科院合肥智能机械研究所 |
| 推荐引用方式 GB/T 7714 | Lei, Kepeng,Sun, Mingtai,Du, Libo,et al. Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe[J]. TALANTA,2017,170(无):314-321. |
| APA | Lei, Kepeng.,Sun, Mingtai.,Du, Libo.,Zhang, Xiaojie.,Yu, Huan.,...&Alsaedi, Ahmed.(2017).Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe.TALANTA,170(无),314-321. |
| MLA | Lei, Kepeng,et al."Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe".TALANTA 170.无(2017):314-321. |
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